钙通道激动剂拜耳K8644对体外培养的大鼠海马CA1区长期增强效应的增强作用。

Enhancement of long-term potentiation by the calcium channel agonist Bayer K8644 in CA1 of the rat hippocampus in vitro.

作者信息

Mulkeen D, Anwyl R, Rowan M J

机构信息

Department of Physiology, Trinity College, Dublin, Ireland.

出版信息

Neurosci Lett. 1987 Oct 5;80(3):351-5. doi: 10.1016/0304-3940(87)90481-2.

DOI:10.1016/0304-3940(87)90481-2

PMID:2446215

Abstract

The effect of the Ca agonist BAY K8644 was studied on long-term potentiation (LTP) of extracellular excitatory postsynaptic potentials in the stratum radiatum of CA1 of the hippocampus in vitro. LTP was evoked by brief trains of high-frequency stimulation applied to the stratum radiatum of CA1. 0.5% Ethanol, the vehicle used to dissolve BAY K8644, reduced LTP from 43% to 15%. An amount of 15 microM BAY K8644, in 0.5% ethanol, enhanced LTP from 13% in the ethanol control to 57%. The Ca channel antagonist verapamil did not alter control LTP, but did inhibit the potentiating action of BAY K8644 on LTP. It is postulated that the enhancement of LTP by BAY K8644 may occur through enhancement of Ca influx through voltage-dependent Ca channels.

摘要

研究了钙激动剂BAY K8644对体外培养的海马CA1区辐射层细胞外兴奋性突触后电位长时程增强（LTP）的影响。通过对CA1区辐射层施加短暂的高频刺激串来诱发LTP。用于溶解BAY K8644的溶剂0.5%乙醇使LTP从43%降至15%。在0.5%乙醇中加入15微摩尔的BAY K8644，可使LTP从乙醇对照组的13%增强至57%。钙通道拮抗剂维拉帕米不改变对照LTP，但能抑制BAY K8644对LTP的增强作用。据推测，BAY K8644对LTP的增强作用可能是通过增强电压依赖性钙通道的钙内流来实现的。