Panin L E
Vopr Med Khim. 1987 Sep-Oct;33(5):96-102.
Cooperative effect of hydrocortisone, adrenaline and high density lipoproteins was found in initiation of the lysosomotropic induction of protein synthesis in liver tissue. Translocation of lysosomes into perinuclear area and transfer of lysosomal hydrolases into nuclei were responsible for specificity of the effect. As a result of consecutive action of carboxylic, serine and thiol proteinases activation of chromatin was induced in nuclei. These processes were inhibited by pepstatin, hordox and iodoacetamide, respectively, as well as by vinblastine--inhibitor of intracellular transport. Actinomycin D inhibited completely the elevated synthesis of RNA and protein under these conditions. The cooperative effect studied appears to be involved in physiological regeneration of liver cells under conditions of their increased functional activity.
在肝组织溶酶体促蛋白合成诱导的起始阶段，发现氢化可的松、肾上腺素和高密度脂蛋白具有协同作用。溶酶体向核周区域的转运以及溶酶体水解酶向细胞核的转移是这种效应特异性的原因。由于羧酸蛋白酶、丝氨酸蛋白酶和巯基蛋白酶的连续作用，细胞核中诱导了染色质的活化。这些过程分别被胃蛋白酶抑制剂、抑肽酶和碘乙酰胺以及细胞内运输抑制剂长春碱所抑制。放线菌素D在这些条件下完全抑制了RNA和蛋白质合成的增加。所研究的协同作用似乎参与了肝细胞在功能活性增加条件下生理再生的过程。
