Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, Osaka, Japan.
J Cell Biochem. 2014 Jul;115(7):1262-8. doi: 10.1002/jcb.24777.
Leukemia inhibitory factor (LIF) is a pleiotropic cytokine that belongs to the interleukin-6 family and is expressed by multiple tissue types. This study analyzed the effect of LIF on osteoblast differentiation using primary murine bone marrow stromal cells (BMSCs). Colony-forming unit-osteoblast formation by BMSCs was significantly suppressed by LIF treatment. To clarify the mechanism underlying the LIF suppressive effect on osteoblast differentiation, we analyzed the downstream signaling pathway of LIF. LIF/signal transducer and activator of transcription 3 (STAT3) signaling induces the expression of suppressor of cytokine signaling 3 (SOCS3). SOCS3 knockdown experiments have previously demonstrated that short-hairpin SOCS3-BMSCs reversed the LIF suppressive effect. Our results demonstrated that LIF suppresses osteoblast differentiation through the LIF/STAT3/SOCS3 signaling pathway.
白血病抑制因子（LIF）是一种多效细胞因子，属于白细胞介素 6 家族，由多种组织类型表达。本研究分析了 LIF 对原代鼠骨髓基质细胞（BMSCs）成骨细胞分化的影响。LIF 处理显著抑制 BMSCs 的集落形成单位-成骨细胞形成。为了阐明 LIF 抑制成骨细胞分化的机制，我们分析了 LIF 的下游信号通路。LIF/信号转导子和转录激活子 3（STAT3）信号诱导细胞因子信号转导抑制因子 3（SOCS3）的表达。SOCS3 短发夹 RNA 实验先前表明，短发夹 SOCS3-BMSCs 逆转了 LIF 的抑制作用。我们的结果表明，LIF 通过 LIF/STAT3/SOCS3 信号通路抑制成骨细胞分化。
