Sun Wei, Guan Qun, Wen Ji, Zhang Qiyao, Yang Weina, Zhang Bin, Cui Wei, Zou Zhiquan, Yu Yan
Reproductive Medical Center, the Second Hospital affiliated to Shandong University of Traditional Chinese Medicine, Jingba Road, Jinan, 250001, People's Republic of China.
J Assist Reprod Genet. 2014 May;31(5):541-7. doi: 10.1007/s10815-014-0177-4. Epub 2014 Jan 26.
The aim of this study was to determine whether altered expression and distribution of calcium- and integrin-binding protein-1 (CIB1) is involved in the pathogenesis of patients with oligoasthenozoospermia.
Sperm samples were obtained from 25 infertile Chinese men who had failed to achieve conception after a period of 1-2 y and had been referred to the Reproductive Laboratory of the second hospital affiliated to the Shandong University of Traditional Chinese Medicine. Participants were divided into two groups: oligoasthenozoospermia (n = 13) and asthenozoospermia (n = 12); as a third group, fertile men (n = 19) were included as controls. The expression levels of mRNA and protein levels of CIB1 and cyclin-dependent kinase 1 (CDK1) were measured using qRT-PCR and western blotting.
mRNA and protein expression levels of CIB1 were decreased in the oligoasthenozoospermia patients. Interestingly mRNA and protein expression levels of CDK1 were increased in the oligoasthenozoospermia patients.
The results of the present study indicate that that CIB1 may be involved in the pathogenesis of oligoasthenozoospermia by the CDK1 signaling pathway.
本研究旨在确定钙整合素结合蛋白-1（CIB1）表达和分布的改变是否参与少弱精子症患者的发病机制。
从25名中国不育男性中获取精液样本，这些男性在1-2年的时间内未能受孕，并被转诊至山东中医药大学第二附属医院生殖实验室。参与者分为两组：少弱精子症组（n = 13）和弱精子症组（n = 12）；作为第三组，纳入19名生育能力正常的男性作为对照。使用qRT-PCR和蛋白质印迹法检测CIB1和细胞周期蛋白依赖性激酶1（CDK1）的mRNA和蛋白质表达水平。
少弱精子症患者中CIB1的mRNA和蛋白质表达水平降低。有趣的是，少弱精子症患者中CDK1的mRNA和蛋白质表达水平升高。
本研究结果表明，CIB1可能通过CDK1信号通路参与少弱精子症的发病机制。
