Singh Pankaj, Vishwakarma Shri P, Singh Ram L
Nutraceutical Laboratory, Department of Biochemistry, Dr. RML Avadh University, Faizabad-, 224 001, UP, India.
J Sci Food Agric. 2014 Sep;94(12):2497-504. doi: 10.1002/jsfa.6585. Epub 2014 Feb 20.
The plant Trigonella foenum-gracecum (TFG) is used as antidiabetic and diuretic. In order to ascertain antioxidant potential of leaf (early and mature) and seed of TFG, total phenolics, free radical scavenging assay, superoxide anion radical scavenging activity, reducing power, lipid peroxidation, ferric thiocyanate assay, hydroxyl radical scavenging activity and DNA damage protective activities were determined. The study was further carried out to assay the antimicrobial activity and HPLC analysis of plant parts.
Ethanol extracts of leaf (early and mature) exhibited a high content of phenolics (54.79 and 41.28 g kg(-1) GAE) when it was compared with seed extract (23.85 g kg(-1) GAE). Results showed that mature TFG leaf extract had the lowest IC50 for the free radical scavenging assay (IC50 = 2.23 mg mL(-1)), superoxide anion radical scavenging activity (IC50 = 2.71 mg mL(-1)), hydroxyl radical scavenging activity (IC50 = 17.30 mg mL(-1)) and highest reducing power (10.14 ascorbic acid equivalents mL(-1)). However, the ethanol seed extract showed the maximum inhibition of lipid peroxidation and the ferric thiocyanate assay. Mature leaf also showed the maximum DNA damage protection activity and higher concentration of phytochemicals.
The results showed that the mature TFG leaf had a higher antioxidant activity, which may be due to the presence of total phenolics. It may be used in herbal drugs or as a nutritional supplement.
胡芦巴（TFG）植物被用作抗糖尿病和利尿剂。为了确定胡芦巴叶（早期和成熟）及种子的抗氧化潜力，测定了总酚含量、自由基清除试验、超氧阴离子自由基清除活性、还原能力、脂质过氧化、硫氰酸铁试验、羟自由基清除活性和DNA损伤保护活性。进一步开展了该植物各部分的抗菌活性测定和高效液相色谱分析。
与种子提取物（23.85 g kg⁻¹ 没食子酸当量）相比，叶（早期和成熟）的乙醇提取物表现出较高的酚含量（54.79和41.28 g kg⁻¹ 没食子酸当量）。结果表明，成熟胡芦巴叶提取物在自由基清除试验（IC50 = 2.23 mg mL⁻¹）、超氧阴离子自由基清除活性（IC50 = 2.71 mg mL⁻¹）、羟自由基清除活性（IC50 = 17.30 mg mL⁻¹）方面具有最低的半数抑制浓度，并且具有最高的还原能力（10.14抗坏血酸当量 mL⁻¹）。然而，乙醇种子提取物对脂质过氧化和硫氰酸铁试验表现出最大抑制作用。成熟叶还表现出最大的DNA损伤保护活性和较高浓度的植物化学物质。
结果表明，成熟胡芦巴叶具有较高的抗氧化活性，这可能归因于总酚的存在。它可用于草药或作为营养补充剂。
