ANTIOXIDANT ACTIVITY AND FT-IR ANALYSIS OF AND LEAF AND SEED METHANOLIC EXTRACTS.

BACKGROUND

(family- Solanaceae), has a long history of being used as herbal medicine. These medicinal effects have been attributed to the phytochemicals present in the plant leaves and seeds, in particular alkaloids, flavonoids and phenolic compounds. The objective of this study was to investigate the methanolic leaf and seed extracts of (DLP-I & DSP-I) and (DLP-M & DSP-M) for their total phenolic, flavonoids and antioxidant properties.

MATERIALS AND METHODS

Determination of total phenolic content and total flavonoid content and antioxidant activity in terms of total antioxidant assay, ABTS assay, DPPH assay and lipid peroxidation inhibiting activity were determined along with the FT-IR (Fourier transform infrared spectroscopy) analysis of the extracts.

RESULTS

The highest total phenolic and total flavonoid content were registered by the leaf extract (70.26 ±1.12 mg GAE/g and 34.24 ± 1.28 mg RE/g respectively). Maximum DPPH radical scavenging activity was exerted by the leaf extract of (IC50 = 146.69 ± 8.46 μg/mL) among the four different methanolic extracts. The highest activity of the ABTS assay was found in leaf extract (IC value = 149.42 ± 13.43 μg/mL) and the highest total antioxidant capacity was found to be present in leaf extract (221.25 ± 1.06 mg AAE/g) whereas seed extract registered the maximum lipid peroxidation inhibition activity (IC = 112 ± 1.30 μg/mL). The FT-IR data also supported the maximum activity in (leaf and seed) extracts.

CONCLUSION

The results thus obtained suggested that the plant possess considerable antioxidant activity over and therefore can be established as a potential source of natural antioxidant.