Islam Md Shoriful, Parvin Mst Shahnaj, Islam Md Ekramul
Dept. of Pharmacy, Islamic University, Kushtia, 7003, Bangladesh.
Dept. of Pharmacy, University of Rajshahi, Rajshahi, 6205, Bangladesh.
Heliyon. 2022 Jan 21;8(1):e08767. doi: 10.1016/j.heliyon.2022.e08767. eCollection 2022 Jan.
Medicinal plants are sources of antioxidant which may protect the body against oxidative stress related diseases and can be used as human food supplements. In this investigation, seeds of (M. R. Almeida & S. M. Almeida) (Fam.-Acanthaceae), a herbaceous plant well known for its medicinal properties, has been examined for antioxidant activity of crude methanolic extract (CME) and its fraction using and assay as well as their protective activity against oxidative damage of DNA and RBC. Total phenolic and flavonoid content have also been estimated using the aluminum chloride colorimetric and Folin-Ciocalteu method. Among the different fractions of CME, Ethyl acetate fraction (EAF) had higher antioxidant activity assay and was selected for antioxidant activity in cadmium intoxicated mice. The EAF showed a significant ( < ) increase in serum catalase and SOD activity compared to the control group. TBARS levels were restored to 17.42 and 19.19 nmol/mg protein, respectively, after treatment with EAF and standard ascorbic acid (AA); compared to the normal group (14.96 nmol/mg protein). Similarly, levels of albumin, bilirubin, uric acid, and alkaline phosphatase were also brought back to normal levels. EAF's protective role against oxidative damage of DNA has shown a significant reduction in destroying of nicked DNA. RBC as a target of oxidation by HO and HOCl, EAF showed inhibition of oxidation in a concentration-dependent manner, compared to standard gallic acid. In this study, we confirmed that EAF could scavenge reactive oxygen species (ROS) thus preventing DNA strand scission and the extract can be used as a functional food or nutraceutical product for health benefits.
药用植物是抗氧化剂的来源，它们可以保护身体免受与氧化应激相关的疾病侵害，并且可以用作人类食品补充剂。在本研究中，对一种以其药用特性而闻名的草本植物（爵床科）的种子进行了研究，检测了其粗甲醇提取物（CME）及其馏分的抗氧化活性，并使用和检测了它们对DNA和红细胞氧化损伤的保护活性。还使用氯化铝比色法和福林-酚试剂法估算了总酚和黄酮含量。在CME的不同馏分中，乙酸乙酯馏分（EAF）在检测中具有较高的抗氧化活性，并被选用于镉中毒小鼠的抗氧化活性研究。与对照组相比，EAF使血清过氧化氢酶和超氧化物歧化酶活性显著（<）增加。在用EAF和标准抗坏血酸（AA）处理后，丙二醛水平分别恢复到17.42和19.19 nmol/mg蛋白质；与正常组（14.96 nmol/mg蛋白质）相比。同样，白蛋白、胆红素、尿酸和碱性磷酸酶水平也恢复到正常水平。EAF对DNA氧化损伤的保护作用表现为切口DNA的破坏显著减少。红细胞作为HO和HOCl氧化的靶点，与标准没食子酸相比，EAF以浓度依赖的方式显示出氧化抑制作用。在本研究中，我们证实EAF可以清除活性氧（ROS），从而防止DNA链断裂，该提取物可以用作具有健康益处的功能性食品或营养保健品。
