浆液性积液中细胞的免疫细胞化学鉴定。技术要点。
Immunocytochemical identification of cells in serous effusions. Technical considerations.
作者信息
Li C Y, Lazcano-Villareal O, Pierre R V, Yam L T
机构信息
Department of Laboratory Medicine, Mayo Clinic, Rochester, Minnesota 55905.
出版信息
Am J Clin Pathol. 1987 Dec;88(6):696-706. doi: 10.1093/ajcp/88.6.696.
Immunocytochemical methods were evaluated in order to find a practical one for cell identification on cytologic preparation of body fluids. The effects of fixatives and Triton X-100 treatment on the preservation of cell-type-specific antigens and cell morphologic characteristics were also examined. The method using the alkaline phosphatase-antialkaline phosphatase (APAAP) complex as the indicator is recommended because of its high specificity and sensitivity. With this method, currently available and potentially useful monoclonal antibodies were examined, and the antibodies that were useful for the identification of normal and neoplastic cells commonly present in body fluids were selected for practical applications.
为了找到一种适用于体液细胞学涂片细胞鉴定的实用免疫细胞化学方法，对其进行了评估。同时还研究了固定剂和Triton X-100处理对细胞类型特异性抗原保存及细胞形态特征的影响。推荐使用以碱性磷酸酶-抗碱性磷酸酶（APAAP）复合物作为指示剂的方法，因为其具有高特异性和高灵敏度。采用该方法，对目前可用的以及可能有用的单克隆抗体进行了检测，并挑选出可用于鉴定体液中常见正常细胞和肿瘤细胞的抗体用于实际应用。
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