School of Biomedical Sciences, The University of Western Australia, Perth, WA 6009, Australia.
Cells. 2021 Dec 31;11(1):127. doi: 10.3390/cells11010127.
I reflect on my experience working with David Y. Mason in the Leukaemia Research Laboratories in the Nuffield Department of Pathology at the University of Oxford in the early 1980s. This was soon after the first monoclonal antibodies had been produced, which led to an exciting and productive time in biological discovery and pathology diagnostics. A specific focus in the laboratory was the development of immunoenzymatic staining methods that would enable monoclonal antibodies to be applied in diagnostic practice. This paper describes the work that led to the performance of immuno-alkaline phosphatase staining on blood and bone marrow smears, the success of which changed leukaemia diagnosis.
我回顾了 20 世纪 80 年代初在牛津大学纳菲尔德病理学系白血病研究实验室与大卫·梅森合作的经历。那时，第一批单克隆抗体刚刚问世，这为生物学发现和病理学诊断带来了令人兴奋和富有成效的时代。实验室的一个特定重点是开发免疫酶染色方法，以使单克隆抗体能够应用于诊断实践。本文描述了导致血液和骨髓涂片上免疫碱性磷酸酶染色的工作，这一成功改变了白血病的诊断。
