Xing Jinyi, Xing Feng, Zhang Chenhua, Zhang Yujie, Wang Nan, Li Yanping, Yang Lijuan, Jiang Chenglan, Zhang Chaoyang, Wen Changhong, Jiang Yunliang
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an, Shandong Province, The People's Republic of China ; College of Life Science, Linyi University, Linyi, Shandong Province, The People's Republic of China.
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an, Shandong Province, The People's Republic of China ; College of Animal Science, Tarim University, Ala'er, Xinjiang Uygur Autonomous Region, The People's Republic of China.
PLoS One. 2014 Jan 23;9(1):e86101. doi: 10.1371/journal.pone.0086101. eCollection 2014.
Porcine reproductive and respiratory syndrome (PRRS) caused by PRRS virus (PRRSV) is an infectious disease characterized by severe reproductive deficiency in pregnant sows, typical respiratory symptoms in piglets, and high mortality rate of piglets. In this study, we employed an Affymetrix microarray chip to compare the gene expression profiles of lung tissue samples from Dapulian (DPL) pigs (a Chinese indigenous pig breed) and Duroc×Landrace×Yorkshire (DLY) pigs after infection with PRRSV. During infection with PRRSV, the DLY pigs exhibited a range of clinical features that typify the disease, whereas the DPL pigs showed only mild signs of the disease. Overall, the DPL group had a lower percentage of CD4(+) cells and lower CD4(+)/CD8(+)ratios than the DLY group (p<0.05). For both IL-10 and TNF-α, the DLY pigs had significantly higher levels than the DPL pigs (p<0.01). The DLY pigs have lower serum IFN-γ levels than the DPL pigs (p<0.01). The serum IgG levels increased slightly from 0 dpi to 7 dpi, and peaked at 14 dpi (p<0.0001). Microarray data analysis revealed 16 differentially expressed (DE) genes in the lung tissue samples from the DLY and DPL pigs (q≤5%), of which LOC100516029 and LOC100523005 were up-regulated in the PRRSV-infected DPL pigs, while the other 14 genes were down-regulated in the PRRSV-infected DPL pigs compared with the PRRSV-infected DLY pigs. The mRNA expression levels of 10 out of the 16 DE genes were validated by real-time quantitative RT-PCR and their fold change was consistent with the result of microarray data analysis. We further analyzed the mRNA expression level of 8 differentially expressed genes between the DPL and DLY pigs for both uninfected and infected groups, and found that TF and USP18 genes were important in underlying porcine resistance or susceptibility to PRRSV.
由猪繁殖与呼吸综合征病毒（PRRSV）引起的猪繁殖与呼吸综合征（PRRS）是一种传染病，其特征为妊娠母猪严重繁殖障碍、仔猪典型呼吸道症状以及仔猪高死亡率。在本研究中，我们使用Affymetrix微阵列芯片比较了大蒲莲猪（DPL，一种中国本土猪种）和杜洛克×长白×大白猪（DLY）感染PRRSV后肺组织样本的基因表达谱。在感染PRRSV期间，DLY猪表现出一系列该疾病的典型临床特征，而DPL猪仅表现出该疾病的轻微症状。总体而言，DPL组的CD4(+)细胞百分比和CD4(+)/CD8(+)比值低于DLY组（p<0.05）。对于IL-10和TNF-α，DLY猪的水平均显著高于DPL猪（p<0.01）。DLY猪的血清IFN-γ水平低于DPL猪（p<0.01）。血清IgG水平从感染后0天（dpi）到7 dpi略有升高，并在14 dpi达到峰值（p<0.0001）。微阵列数据分析显示，DLY和DPL猪肺组织样本中有16个差异表达（DE）基因（q≤5%），其中LOC100516029和LOC100523005在感染PRRSV的DPL猪中上调，而与感染PRRSV的DLY猪相比，其他14个基因在感染PRRSV的DPL猪中下调。通过实时定量RT-PCR验证了16个DE基因中10个基因的mRNA表达水平，其倍数变化与微阵列数据分析结果一致。我们进一步分析了未感染和感染组中DPL和DLY猪之间8个差异表达基因的mRNA表达水平，发现TF和USP18基因在猪对PRRSV的抗性或易感性中起重要作用。
