Gravel M, Melançon P, Brakier-Gingras L
Départment de Biochimie, Université de Montréal, Québec, Canada.
Biochemistry. 1987 Sep 22;26(19):6227-32. doi: 10.1021/bi00393a041.
[3H]Dihydrostreptomycin was cross-linked to the 30S ribosomal subunit from Escherichia coli with the bifunctional reagent nitrogen mustard. The cross-linking primarily involved the 16S RNA. To localize the site of cross-linking of streptomycin to the 16S RNA, we hybridized RNA labeled with streptomycin to restriction fragments of the 16S RNA gene. Labeled RNA hybridized to DNA fragments corresponding to bases 892-917 and bases 1394-1415. These two segments of the ribosomal RNA must be juxtaposed in the ribosome, since there is a single binding site for streptomycin. This region has been implicated both in the decoding site and in the binding of initiation factor IF-3, indicating its functional importance.
用双功能试剂氮芥将[3H]二氢链霉素与大肠杆菌的30S核糖体亚基交联。这种交联主要涉及16S RNA。为了确定链霉素与16S RNA的交联位点，我们将用链霉素标记的RNA与16S RNA基因的限制性片段杂交。标记的RNA与对应于碱基892 - 917和碱基1394 - 1415的DNA片段杂交。核糖体RNA的这两个片段在核糖体中必定是并列的，因为链霉素有一个单一的结合位点。该区域与解码位点以及起始因子IF - 3的结合均有关联，表明了其功能重要性。
