链霉素或双氢链霉素与16S RNA或30S核糖体亚基结合的效应。
The effects of streptomycin or dihydrostreptomycin binding to 16S RNA or to 30S ribosomal subunits.
作者信息
Garvin R T, Biswas D K, Gorini L
出版信息
Proc Natl Acad Sci U S A. 1974 Oct;71(10):3814-8. doi: 10.1073/pnas.71.10.3814.
Abstract
Evidence is presented suggesting that streptomycin binds to 16S RNA or to 30S ribosomal subunits at the same topographical site located on the RNA chain. The equally bactericidal dihydrostreptomycin binds to the same site as streptomycin but with lower affinity. The effect of drug binding to 16S RNA (measured by reconstitution inhibition) is readily reversible, while that of drug binding to 30S subunits (measured by misreading) persists after removal of the drug. Binding of the drug is not a necessary and sufficient reason for killing.
摘要
有证据表明，链霉素与16S RNA或30S核糖体亚基结合于RNA链上相同的拓扑学位点。同样具有杀菌作用的双氢链霉素与链霉素结合于同一位点，但亲和力较低。药物与16S RNA结合的效应（通过重组抑制来测定）很容易逆转，而药物与30S亚基结合的效应（通过错读来测定）在药物去除后仍会持续。药物的结合并非杀菌的必要且充分原因。
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