Department of Chemical Physiology, The Scripps Research Institute , North Torrey Pines Road, Suite 11, La Jolla, California 92037, United States.
J Proteome Res. 2014 Mar 7;13(3):1545-59. doi: 10.1021/pr401075f. Epub 2014 Jan 27.
Cryptococcus neoformans, a pathogenic yeast, causes meningoencephalitis, especially in immunocompromised patients, leading in some cases to death. Microbes in biofilms can cause persistent infections, which are harder to treat. Cryptococcal biofilms are becoming common due to the growing use of brain valves and other medical devices. Using shotgun proteomics we determine the differences in protein abundance between biofilm and planktonic cells. Applying bioinformatic tools, we also evaluated the metabolic pathways involved in biofilm maintenance and protein interactions. Our proteomic data suggest general changes in metabolism, protein turnover, and global stress responses. Biofilm cells show an increase in proteins related to oxidation-reduction, proteolysis, and response to stress and a reduction in proteins related to metabolic process, transport, and translation. An increase in pyruvate-utilizing enzymes was detected, suggesting a shift from the TCA cycle to fermentation-derived energy acquisition. Additionally, we assign putative roles to 33 proteins previously categorized as hypothetical. Many changes in metabolic enzymes were identified in studies of bacterial biofilm, potentially revealing a conserved strategy in biofilm lifestyle.
新型隐球菌是一种致病性酵母，可引起脑膜脑炎，特别是在免疫功能低下的患者中，在某些情况下可导致死亡。生物膜中的微生物可引起持续性感染，这更难治疗。由于脑室外引流管和其他医疗器械的广泛使用，新型隐球菌生物膜越来越常见。我们使用鸟枪法蛋白质组学确定生物膜和浮游细胞之间蛋白质丰度的差异。应用生物信息学工具，我们还评估了参与生物膜维持和蛋白质相互作用的代谢途径。我们的蛋白质组学数据表明代谢、蛋白质周转和全局应激反应的普遍变化。生物膜细胞中与氧化还原、蛋白水解以及应激反应相关的蛋白质增加，而与代谢过程、运输和翻译相关的蛋白质减少。检测到丙酮酸利用酶的增加，表明从三羧酸循环到发酵衍生的能量获取的转变。此外，我们将 33 种先前归类为假设的蛋白质赋予了假定的作用。在细菌生物膜的研究中发现了许多代谢酶的变化，这可能揭示了生物膜生活方式的保守策略。
