Hupkes Marlinda, Sotoca Ana M, Hendriks José M, van Zoelen Everardus J, Dechering Koen J
Department of Cell & Applied Biology, Faculty of Science, Nijmegen Centre for Molecular Life Sciences (NCMLS), Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ, Nijmegen, The Netherlands.
BMC Mol Biol. 2014 Jan 27;15:1. doi: 10.1186/1471-2199-15-1.
MicroRNAs (miRNAs) are a family of small, non-coding single-stranded RNA molecules involved in post-transcriptional regulation of gene expression. As such, they are believed to play a role in regulating the step-wise changes in gene expression patterns that occur during cell fate specification of multipotent stem cells. Here, we have studied whether terminal differentiation of C2C12 myoblasts is indeed controlled by lineage-specific changes in miRNA expression.
Using a previously generated RNA polymerase II (Pol-II) ChIP-on-chip dataset, we show differential Pol-II occupancy at the promoter regions of six miRNAs during C2C12 myogenic versus BMP2-induced osteogenic differentiation. Overexpression of one of these miRNAs, miR-378, enhances Alp activity, calcium deposition and mRNA expression of osteogenic marker genes in the presence of BMP2.
Our results demonstrate a previously unknown role for miR-378 in promoting BMP2-induced osteogenic differentiation.
微小RNA（miRNA）是一类小的非编码单链RNA分子家族，参与基因表达的转录后调控。因此，它们被认为在调节多能干细胞细胞命运特化过程中发生的基因表达模式的逐步变化中发挥作用。在此，我们研究了C2C12成肌细胞的终末分化是否确实受miRNA表达的谱系特异性变化控制。
利用先前生成的RNA聚合酶II（Pol-II）芯片染色质免疫沉淀数据集，我们发现在C2C12成肌分化与BMP2诱导的成骨分化过程中，六种miRNA的启动子区域存在不同的Pol-II占据情况。其中一种miRNA，miR-378，在BMP2存在的情况下过表达可增强碱性磷酸酶（Alp）活性、钙沉积和成骨标记基因的mRNA表达。
我们的结果证明了miR-378在促进BMP2诱导的成骨分化中具有先前未知的作用。
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