Shang Jing, Liu Jia, He Mu, Shang Erxin, Zhang Li, Shan Mingqiu, Yao Weifeng, Yu Bing, Yao Yingzhi, Ding Anwei
Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, 138 Xianlin Road, Nanjing 210046, China.
Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, 138 Xianlin Road, Nanjing 210046, China.
J Pharm Biomed Anal. 2014 Apr;92:26-34. doi: 10.1016/j.jpba.2013.12.019. Epub 2013 Dec 27.
Blood heat and hemorrhage (BHH) syndrome is the most common bleeding disease in clinic. In this study, a rat model with BHH syndrome was built for the first time. Biochemical study showed the intrinsic coagulation pathways and the platelet aggregation rate in the rat model were inhibited, while extrinsic pathway of coagulation cascade was activated. An UHPLC/Q-TOF MS combined with orthogonal partial least squares-discriminant analysis (OPLS-DA) was employed to construct plasma metabolic profiling of the rat model with BHH syndrome. Twenty-four unique metabolites were identified, which were involved in glycerophospholipid metabolism, arachidonic acid metabolism, fatty acid metabolism, amino acid metabolism and cholic acid metabolism. In the end, we concluded that bleeding mechanism of the rat with BHH syndrome may be associated with augmenting blood viscosity, inhibiting platelet aggregation and intrinsic coagulation pathways.
血热出血（BHH）证是临床最常见的出血性疾病。本研究首次构建了BHH证大鼠模型。生化研究表明，该大鼠模型的内源性凝血途径及血小板聚集率受到抑制，而凝血级联反应的外源性途径被激活。采用超高效液相色谱/四极杆飞行时间质谱联用技术结合正交偏最小二乘法判别分析（OPLS-DA）构建BHH证大鼠模型的血浆代谢谱。共鉴定出24种独特的代谢物，它们参与甘油磷脂代谢、花生四烯酸代谢、脂肪酸代谢、氨基酸代谢和胆汁酸代谢。最后，我们得出结论，BHH证大鼠的出血机制可能与血液粘度增加、血小板聚集及内源性凝血途径受抑制有关。
