Department of Traditional Chinese Medicine, Guangdong Pharmaceutical University, The Key Unit of Chinese Medicine Digitalization Quality Evaluation of State Administration of Traditional Chinese Medicine, The Research Center for Quality Engineering Technology of Traditional Chinese Medicine, Guangzhou, China.
Guangzhou Medical University Cancer Hospital and Institute, Guangzhou, Guangdong, China.
J Pharm Biomed Anal. 2019 May 30;169:151-158. doi: 10.1016/j.jpba.2019.02.044. Epub 2019 Feb 28.
Moutan Cortex charcoal (MCC), the processed root bark of Paeonia suff ;ruticosa Andrews (Paeoniaceae), is a kind of traditional Chinese medicine (TCM) and has been used for treating blood-heat and hemorrhage(BHH)syndrome in China for thousands of years. In order to explore potential metabolic mechanism, H NMR-based metabonomics technique was applied to evaluate the effect of MCC on metabolic changes in plasma and urine of BHH rat models. Serum and urine samples were obtained from male SD rats with normal group, model group and MCC group for study. Based on H NMR spectra obtained from plasma and urine samples, principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA) models were capable of distinguishing the three group. And the 13 pharmacodynamic biomarkers of MCC were identified in the plasma and urine. The results showed that BHH induced great metabolic disorders in plasma and urine metabolisms. However, MCC could reverse the imbalanced metabolites by alanine, aspartate and glutamate metabolism and citrate cycle (TCA cycle) pathway, and its effect was also confirmed by the general signs and pharmacodynamics assessments. The results indicated that NMR-based metabolomic profiling method is sensitive and specific enough to evaluate the MCC efficacy and mechanism of action on BHH syndromes.
牡丹皮炭（MCC），是毛茛科植物牡丹（Paeonia suffruticosa Andrews）的炮制根皮，是一种传统中药，在中国已有数千年用于治疗血热和出血（BHH）综合征。为了探索潜在的代谢机制，采用基于 1 H NMR 的代谢组学技术来评估 MCC 对 BHH 大鼠模型血浆和尿液代谢变化的影响。从雄性 SD 大鼠中获得血清和尿液样本，分为正常组、模型组和 MCC 组进行研究。基于从血浆和尿液样本中获得的 1 H NMR 图谱，主成分分析（PCA）和正交偏最小二乘判别分析（OPLS-DA）模型能够区分这三组。并在血浆和尿液中鉴定出 13 种 MCC 的药效生物标志物。结果表明，BHH 引起了血浆和尿液代谢的严重代谢紊乱。然而，MCC 可以通过丙氨酸、天冬氨酸和谷氨酸代谢和柠檬酸循环（TCA 循环）途径来逆转失衡的代谢物，其作用也通过一般体征和药效学评估得到了证实。结果表明，基于 NMR 的代谢组学分析方法足够灵敏和特异，可用于评估 MCC 对 BHH 综合征的疗效和作用机制。
