Zhang Meiling, Cheng Jinjun, Luo Juan, Li Changxiang, Hou Tingting, Zhao Yan, Wang Yaoxian, Qu Huihua, Kong Hui
School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China.
Key Laboratory of Chinese Internal Medicine of the Ministry of Education, Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine, Beijing, China.
Front Pharmacol. 2023 Aug 10;14:1118550. doi: 10.3389/fphar.2023.1118550. eCollection 2023.
As the charcoal processing product of (SR), SR Carbonisata (SRC) has been clinically used as a cooling blood and hemostatic agent for thousands of years. However, the underlying active ingredients and mechanism of SRC still remained unspecified. In this study, SRC derived carbon dots (SRC-CDs) were extracted and purified from the aqueous solution of SRC, followed by physicochemical property assessment by series of technologies. The cooling blood and hemostatic effects of SRC-CDs were further evaluated via a blood-heat and hemorrhage (BHH) rat model. Results showed that the diameters of obtained fluorescent SRC-CDs ranged from 5.0 nm to 10.0 nm and possessed functional group-rich surfaces. Additionally, the as-prepared SRC-CDs showed remarkable cooling blood and hemostasis effects in BHH model, mainly manifested by significant improvement of elevated rectal temperature, inflammatory cytokines (TNF-α, IL-6, and IL-1β) levels, as well as protein expressions of myD88 and NF-κB p65, abnormal coagulation parameters (elevated APTT and FIB), hemogram parameters (RBC, HGB, and HCT), and histopathological changes in lung and gastric tissues. This study, for the first time, demonstrated that SRC-CDs were the cooling blood and hemostatic active components of SRC, which could inhibit the release of inflammatory cytokines by regulating myD88/NF-κB signaling pathway, and activating the fibrin system and endogenous coagulation pathway. These results not only provide a new perspective for the study of active ingredients of carbonized herbs represented by SRC, but also lay an experimental foundation for the development of next-generation nanomedicines.
作为（SR）的炭加工产物，煅炭（SRC）在临床上作为凉血止血剂已使用了数千年。然而，SRC的潜在活性成分和作用机制仍不明确。在本研究中，从SRC水溶液中提取并纯化了SRC衍生的碳点（SRC-CDs），随后通过一系列技术对其进行了理化性质评估。通过血热出血（BHH）大鼠模型进一步评估了SRC-CDs的凉血止血作用。结果表明，所获得的荧光SRC-CDs直径范围为5.0nm至10.0nm，表面富含官能团。此外，所制备的SRC-CDs在BHH模型中显示出显著的凉血止血作用，主要表现为直肠温度升高、炎症细胞因子（TNF-α、IL-6和IL-1β）水平、myD88和NF-κB p65的蛋白表达、异常凝血参数（APTT和FIB升高）、血常规参数（RBC、HGB和HCT）以及肺和胃组织的组织病理学变化均得到显著改善。本研究首次证明SRC-CDs是SRC的凉血止血活性成分，其可通过调节myD88/NF-κB信号通路抑制炎症细胞因子的释放，并激活纤维蛋白系统和内源性凝血途径。这些结果不仅为以SRC为代表的炭类中药活性成分的研究提供了新的视角，也为下一代纳米药物的开发奠定了实验基础。
