Maheshwari Richa, Panigrahi Gatikrushna, Angappan K
Department of Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, 641 003, Tamil Nadu, India,
Mol Biol Rep. 2014;41(4):2635-44. doi: 10.1007/s11033-014-3122-9. Epub 2014 Jan 28.
The present study was carried out to find out the variations present in different isolates of yellow mosaic virus (YMV) causing yellow mosaic disease of pulses in southern parts of India. The coat protein gene of YMV was amplified using gene specific and deng universal primers with DNA isolated from YMV infected samples. Further, cloning and DNA sequencing of CP gene was carried out. CP gene decrypt sequences revealed that YMV infected samples of Black gram, Cowpea and Green gram were similar to the MYMV-Tamil Nadu isolates. Whereas the YMV infected sample of Horse gram was found to be similar with HYMV. Hence, in the present study, two distinct YMV infecting pulses in Tamil Nadu (MYMV and HYMV species) were identified and it was observed that there exists considerable genetic variation among these species. In addition, Cowpea crop which was earlier supposed not to be susceptible for YMV infection also showed the presence of this virus similar to the MYMV. Overall, the findings of the present study indicate that the CP region is efficient enough to provide a simple, rapid, and reliable method for early detection of YMV infections in pulses, which would help to develop proper management strategies to control these viruses.
本研究旨在查明在印度南部引起豆类黄花叶病的黄花叶病毒（YMV）不同分离株中存在的变异情况。使用基因特异性引物和通用引物，从感染YMV的样本中分离出DNA，对YMV的外壳蛋白基因进行扩增。进一步对CP基因进行克隆和DNA测序。CP基因解密序列显示，感染YMV的黑豆、豇豆和绿豆样本与MYMV - 泰米尔纳德邦分离株相似。而感染YMV的木豆样本则与HYMV相似。因此，在本研究中，鉴定出了在泰米尔纳德邦感染豆类的两种不同的YMV（MYMV和HYMV物种），并且观察到这些物种之间存在相当大的遗传变异。此外，先前认为不易感染YMV的豇豆作物也显示出存在与MYMV相似的这种病毒。总体而言，本研究结果表明，CP区域足以提供一种简单、快速且可靠的方法来早期检测豆类中的YMV感染，这将有助于制定适当的管理策略来控制这些病毒。
