School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, People's Republic of China.
Environ Sci Pollut Res Int. 2014 May;21(9):6069-75. doi: 10.1007/s11356-014-2564-x. Epub 2014 Jan 28.
To characterize Streptomyces isolated from cattle feces for converting lignocellulose into reducing sugars, five Streptomyces strains were screened. All the strains could convert lignocellulose into reducing sugars. The strain A16 accumulate 3.3-folds more reducing sugars on cottonseed shells treated with ethanol than without the treatment (P < 0.05). The five strains did not accumulate more reducing sugars on rice straws and wheat brans than those on cottonseed shells. Compared with A10 alone, the microbial combination of F1 + A10 accumulated 19, 61, and 25 % less reducing sugars on cottonseed shell, rice straw, and wheat bran than those by A10 solely, respectively (P < 0.05). Further studies indicated that the activities of avicelase and xylanase were not correlated with the reducing sugar amount accumulated by the test strains. Strain A7 could produce more cellular lipids with xylose and glucose as the sole carbon sources. This study shows the potential for Streptomyces strains from herbivore feces to convert lignocelluloses into lipids and reducing sugars for fuel production.
为了研究从牛粪便中分离的链霉菌将木质纤维素转化为还原糖的特性，筛选了 5 株链霉菌。所有菌株均能将木质纤维素转化为还原糖。菌株 A16 在经乙醇处理的棉籽壳上积累的还原糖比未经处理的棉籽壳多 3.3 倍（P<0.05）。与棉籽壳相比，这 5 株菌在稻草和麦麸上并没有积累更多的还原糖。与单独的 A10 相比，F1+A10 的微生物组合在棉籽壳、稻草和麦麸上积累的还原糖分别比单独的 A10 少 19%、61%和 25%（P<0.05）。进一步的研究表明，滤纸酶和木聚糖酶的活性与试验菌株积累的还原糖量不相关。菌株 A7 可以利用木糖和葡萄糖作为唯一碳源产生更多的细胞脂质。本研究表明，食草动物粪便中的链霉菌具有将木质纤维素转化为脂质和还原糖用于燃料生产的潜力。
