School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, China.
Lett Appl Microbiol. 2013 Sep;57(3):200-5. doi: 10.1111/lam.12096. Epub 2013 May 20.
An actinomycete producing oil-like mixtures was isolated and characterized. The strain was isolated from sheep faeces and identified as Streptomyces sp. S161 based on 16S rRNA gene sequence analysis. The strain showed cellulase and xylanase activities. The (1) H nuclear magnetic resonance (NMR) spectra of the mixtures showed that the mixtures were composed of fatty acid methyl esters (52·5), triglycerides (13·7) and monoglycerides (9·1) (mol.%). Based on the gas chromatography-mass spectrometry (GC-MS) analysis, the fatty acid methyl esters were mainly composed of C14-C16 long-chain fatty acids. The results indicated that Streptomyces sp. S161 could produce fatty acid methyl esters (FAME) directly from starch. To our knowledge, this is the first isolated strain that can produce biodiesel (FAME) directly from starch.
从羊粪便中分离得到一株产油样混合物的放线菌，并对其进行了鉴定。根据 16S rRNA 基因序列分析，该菌株被鉴定为链霉菌属 S161 株。该菌株表现出纤维素酶和木聚糖酶活性。混合物的 (1)H 核磁共振 (NMR) 谱表明，混合物由脂肪酸甲酯（52.5%）、甘油三酯（13.7%）和单甘酯（9.1%）（摩尔%）组成。根据气相色谱-质谱联用分析（GC-MS），脂肪酸甲酯主要由 C14-C16 长链脂肪酸组成。结果表明，链霉菌属 S161 株可以直接从淀粉中生产脂肪酸甲酯（FAME）。据我们所知，这是第一株可以直接从淀粉中生产生物柴油（FAME）的分离菌株。
