Departments of Chemical Engineering and of Bacteriology and Biochemistry, University of Idaho, Moscow, Idaho 83843.
Appl Environ Microbiol. 1989 May;55(5):1165-8. doi: 10.1128/aem.55.5.1165-1168.1989.
Streptomyces viridosporus T7A and S. badius 252 were grown in 1 to 2% (wt/vol) slurry cultures with mineral salts solution containing 0.6% yeast extract and 100/200 mesh ground and extracted corn lignocellulose at 37 degrees C. Enzyme activities rapidly increased in the first 3 to 4 days and then declined and remained at a relatively constant level. Concentrations of endoglucanase and xylanase produced by S. badius were lower than those produced by S. viridosporus. However, the lignin-peroxidase peak concentration was threefold higher than with S. viridosporus and was obtained at 9 to 10 days of incubation. By polyacrylamide gel analysis, it was determined that peroxidases from both species consisted of four enzymes, with only one, the lignin peroxidase, having high activity. A culture pH of 8.5 was preferable for lignocellulose degradation by S. badius.
变色链霉菌 T7A 和黄孢原毛平革菌 252 在含有 0.6%酵母提取物和 100/200 目粉碎和提取的玉米木质纤维素的无机盐溶液中以 1%至 2%（wt/vol）的淤浆培养物中生长，在 37°C 下培养。酶活性在前 3 到 4 天迅速增加，然后下降并保持在相对稳定的水平。黄孢原毛平革菌产生的内切葡聚糖酶和木聚糖酶的浓度低于变色链霉菌产生的酶浓度。然而，木质素过氧化物酶的峰值浓度是变色链霉菌的三倍，在培养 9 到 10 天时达到。通过聚丙烯酰胺凝胶分析，确定两种菌的过氧化物酶均由四种酶组成，只有一种木质素过氧化物酶具有高活性。黄孢原毛平革菌降解木质纤维素的最佳培养 pH 值为 8.5。
