Beutelmann P
Institut für Allgemeine Botanik der Universität, D-6500, Mainz, Federal Republic of Germany.
Planta. 1973 Jun;112(2):181-90. doi: 10.1007/BF00388588.
When callus cells derived from the sporogon of the hybrid Funaria hygrometrica x Physcomitrium piriforme are supplied with adenine-8-(14)C, they produce a labelled cytokinin which has the same chromatographic behavior as N(6)-γγ-(dimethylallyl)aminopurine. The cytokinin is the first radioactive product that can be detected in the culture medium. It is formed as long as labelled adenine is available. When callus cells are grown in an optimum culture medium containing amino acids, about 10% of the radioactivity supplied as adenine is found in the cytokinin. When the cells are grown in a medium without amino acids, the RNA-content of the cells and the total yield of cytokinin decrease, but about 18% of the radioactivity is taken up into RNA as adenine and guanine, which are both degraded to allantoin and urea but not converted to cytokinin. Free guanine is converted neither to adenine nor to cytokinin.Weak cytokinin activity can be detected in hydrolysates of sRNA, but no radioactive cytokinin can be isolated from sRNA of adenine-labelled callus cells. it is assumed that free cytokinin is not a degradation product of tRNA.
当用腺嘌呤-8-(14)C 处理源自杂交种大灰藓×梨蒴立碗藓孢子体的愈伤组织细胞时，它们会产生一种标记的细胞分裂素，其色谱行为与 N(6)-γγ-(二甲基烯丙基)氨基嘌呤相同。这种细胞分裂素是在培养基中可检测到的第一种放射性产物。只要有标记的腺嘌呤，它就会形成。当愈伤组织细胞在含有氨基酸的最佳培养基中生长时，作为腺嘌呤提供的放射性中约有 10%存在于细胞分裂素中。当细胞在不含氨基酸的培养基中生长时，细胞的 RNA 含量和细胞分裂素的总产量会降低，但约 18%的放射性会以腺嘌呤和鸟嘌呤的形式被吸收到 RNA 中，它们都会降解为尿囊素和尿素，但不会转化为细胞分裂素。游离鸟嘌呤既不会转化为腺嘌呤也不会转化为细胞分裂素。在 sRNA 的水解产物中可检测到微弱的细胞分裂素活性，但从用腺嘌呤标记的愈伤组织细胞的 sRNA 中无法分离出放射性细胞分裂素。据推测，游离细胞分裂素不是 tRNA 的降解产物。
