Qi Wei, Cai Peng, Yuan Wenjing, Wang Hua
Key Laboratory of Life-Organic Analysis, School of Chemistry and Chemical Engineering, Qufu Normal University, 57 Jingxuan West Road, Qufu Shandong, 273165, China; Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, School of Chemistry and Chemical Engineering, Qufu Normal University, 57 Jingxuan West Road, Qufu Shandong, 273165, China.
J Biomed Mater Res A. 2014 Nov;102(11):4071-7. doi: 10.1002/jbm.a.35094. Epub 2014 Feb 4.
For polyelectrolyte multilayers (PEMs) assembled by the layer-by-layer (LbL) assembly technique, their nanostructure and properties can be governed by many parameters during the building process. Here, it was demonstrated that the swelling of the PEMs containing poly(diallyldimethylammonium chloride) (PDDA) and poly(sodium 4-styrenesulfonate) (PSS) in cell culture media could be tuned with changing supporting salt solutions during the assembly process. Importantly, the influence of the PEMs assembled in different salt solutions on NIH-3T3 cell adhesion was observable. Specifically, the cells could possess a higher affinity for the films assembled in low salt concentration (i.e. 0.15M NaCl) or no salt, the poorly swelling films in cell culture media, which was manifested by the large cell spreading area and focal adhesions. In contrast, those were assembled in higher salt concentration, highly swelling films in cell culture media, were less attractive for the fibroblasts. As a result, the cell adhesion behaviors may be manipulated by tailoring the physicochemical properties of the films, which could be performed by changing the assembly conditions such as supporting salt concentration. Such a finding might promise a great potential in designing desired biomaterials for tissue engineering and regenerative medicine.
对于通过层层（LbL）组装技术组装的聚电解质多层膜（PEMs），其纳米结构和性能在构建过程中会受到许多参数的影响。在此，研究表明，在组装过程中，通过改变支撑盐溶液，可以调节含有聚二烯丙基二甲基氯化铵（PDDA）和聚（4-苯乙烯磺酸钠）（PSS）的PEMs在细胞培养基中的溶胀情况。重要的是，可以观察到在不同盐溶液中组装的PEMs对NIH-3T3细胞黏附的影响。具体而言，细胞对在低盐浓度（即0.15M NaCl）或无盐条件下组装的膜具有更高的亲和力，这些膜在细胞培养基中溶胀性较差，表现为细胞铺展面积大且有粘着斑。相比之下，那些在高盐浓度下组装的膜，即在细胞培养基中溶胀性高的膜，对成纤维细胞的吸引力较小。因此，可以通过调整膜的物理化学性质来控制细胞黏附行为，这可以通过改变组装条件如支撑盐浓度来实现。这一发现可能为设计用于组织工程和再生医学的理想生物材料带来巨大潜力。
