MOE of Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, People's Republic of China.
J R Soc Interface. 2012 Dec 7;9(77):3455-68. doi: 10.1098/rsif.2012.0546. Epub 2012 Aug 15.
The cell migration plays a crucial role in a variety of physiological and pathological processes and can be regulated by the cell-substrate interactions. We found previously that the poly(sodium 4-styrenesulphonate) (PSS)/poly(diallyldimethylammonium) chloride (PDADMAC) multilayers post-treated in 1-5 M NaCl solutions result in continuous changes of their physico-chemical properties such as thickness, chemical composition, surface charge, swelling ratio and wettability. In this study, the responses of human smooth muscle cells (SMCs) on these salt-treated multilayers, particularly the governing factors of cellular migration that offer principles for designing therapeutics and implants, were disclosed. The cell migration rate was slowest on the 3 M NaCl-treated multilayers, which was comparable with that on tissue culture plates, but it was highest on 5 M NaCl-treated multilayers. To elucidate the intrinsic mechanisms, cell adhesion, proliferation, adhesion and related gene expressions were further investigated. The SMCs preferred to attach, spread and proliferate on the PSS-dominated surfaces with well-organized focal adhesion and actin fibres, especially on the 3 M NaCl-treated multilayers, while were kept round and showed low viability on the PDADMAC-dominated surfaces. The relative mRNA expression levels of adhesion-related genes such as fibronectin, laminin and focal adhesion kinase, and migration-related genes such as myosin IIA and Cdc42 were compared to explain the different cellular behaviours. These results reveal that the surface chemistry and the swelling of the salt-treated multilayers govern the cell migration behaviours.
细胞迁移在多种生理和病理过程中起着至关重要的作用，可以通过细胞-基质相互作用来调节。我们之前发现，在 1-5 M NaCl 溶液中后处理的聚（4-苯乙烯磺酸钠）（PSS）/聚二烯丙基二甲基氯化铵（PDADMAC）多层膜会导致其物理化学性质（如厚度、化学成分、表面电荷、溶胀比和润湿性）发生连续变化。在这项研究中，揭示了人平滑肌细胞（SMCs）对这些盐处理多层膜的反应，特别是细胞迁移的控制因素，这为治疗和植入物的设计提供了原理。细胞迁移率在 3 M NaCl 处理的多层膜上最慢，与组织培养板上的迁移率相当，但在 5 M NaCl 处理的多层膜上最快。为了阐明内在机制，进一步研究了细胞黏附、增殖、黏附和相关基因表达。SMCs 更喜欢附着、伸展和增殖在具有组织良好的焦点黏附和肌动蛋白纤维的 PSS 主导表面上，尤其是在 3 M NaCl 处理的多层膜上，而在 PDADMAC 主导表面上则保持圆形且活力较低。比较了黏附相关基因（如纤连蛋白、层粘连蛋白和黏着斑激酶）和迁移相关基因（如肌球蛋白 IIA 和 Cdc42）的相对 mRNA 表达水平，以解释不同的细胞行为。这些结果表明，表面化学和盐处理多层膜的溶胀控制着细胞迁移行为。
