大肠杆菌分裂周期中前体池大小的变化：线性细胞生长的进一步证据。
Variation in precursor pool size during the division cycle of Escherichia coli: further evidence for linear cell growth.
作者信息
Kubitschek H E, Pai S R
机构信息
Biological, Environmental, and Medical Research Division, Argonne National Laboratory, Illinois 60439.
出版信息
J Bacteriol. 1988 Jan;170(1):431-5. doi: 10.1128/jb.170.1.431-435.1988.
Abstract
The magnitudes of several pools of radioactively labeled precursors for RNA and protein synthesis were determined as a function of cell age during the division cycle of Escherichia coli 15 THU. Uracil, histidine, and methionine pools increased from low initial values for cells at birth to maxima during midcycle and then subsided again. These pools were small or nonexistent at the beginning and the end of the cycle, and their average values during the cycle were less than 4% of the total cellular radioactivity. The results are consistent with a linear pattern of growth for cells during the division cycle and provide strong evidence against exponential or bilinear growth of E. coli cells.
摘要
在大肠杆菌15 THU的分裂周期中，测定了几个用于RNA和蛋白质合成的放射性标记前体池的大小与细胞年龄的函数关系。尿嘧啶、组氨酸和蛋氨酸池从细胞出生时的低初始值增加到周期中期的最大值，然后再次下降。这些池在周期开始和结束时很小或不存在，并且它们在周期中的平均值不到细胞总放射性的4%。结果与细胞在分裂周期中的线性生长模式一致，并为反对大肠杆菌细胞的指数或双线性生长提供了有力证据。
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