Weissinger Eva M, Mullen William, Albalat Amaya
Department of Hematology, Hannover Medical School, Hemostasis and Oncology, Hannover, Germany.
Methods Mol Biol. 2014;1109:293-306. doi: 10.1007/978-1-4614-9437-9_15.
Complex biological samples hold significant information on the health status and development of disease. Approximately 22,000 human genes give rise to more than 400,000 proteins as functional entities (Anderson and Anderson, Electrophoresis 19:1853-1861, 1998). Thus, the proteome provides a much richer source of information than the genome for describing the state of health or disease of humans. The composition of body fluids comprises a rich source of information on changes of protein and peptide expression. Here we describe the application of capillary electrophoresis (CE) coupled online to an electrospray-ionization time-of-flight mass spectrometer (ESI-TOF-MS) to analyze human urine for the identification of biomarkers specific for complications after allogeneic hematopoietic stem cell transplantation (Kaiser et al. Blood 104:340-349, 2004; Weissinger et al. Blood 109:5511-5519, 2007). In addition, we describe methods for the sequencing of native proteins/peptides, necessary for the identification of possible new therapeutic targets.
复杂的生物样本蕴含着有关健康状况和疾病发展的重要信息。大约22000个人类基因产生了超过400000种作为功能实体的蛋白质（安德森和安德森，《电泳》19:1853 - 1861，1998年）。因此，对于描述人类的健康或疾病状态而言，蛋白质组比基因组提供了更为丰富的信息来源。体液的成分包含了有关蛋白质和肽表达变化的丰富信息来源。在此，我们描述了将毛细管电泳（CE）与电喷雾电离飞行时间质谱仪（ESI - TOF - MS）在线联用，以分析人类尿液，从而鉴定异基因造血干细胞移植后并发症特异性生物标志物的应用（凯泽等人，《血液》104:340 - 349，2004年；魏辛格等人，《血液》109:5511 - 5519，2007年）。此外，我们还描述了鉴定可能的新治疗靶点所必需的天然蛋白质/肽测序方法。
