Harms H, Prieß I
Institut für Biochemie des Bodens und Isotopenlaboratorium der Forschungsanstalt für Landwirtschaft, D-3300, Braunschweig-Völkenrode, Bundesrepublik Deutschland.
Planta. 1973 Dec;109(4):307-15. doi: 10.1007/BF00387099.
Various methoxybenzoic acids (anisic, veratric and 3,4,5-trimethoxybenzoic acid) labelled specifically in para and meta methoxyl groups as well as the corresponding 4-hydroxybenzoic acids were added to the nutrient solution of sterile cultures of wheat seedlings.The experiments show that the O-demethylation of benzoic acids is specific for para methoxy groups. meta-O-Methyl carbon atoms appeared only to a very low extent as CO2 and no products formed by demethylation of these groups could be isolated.The products formed by O-demethylation of the para methoxyl groups could be identified as p-hydroxybenzoic acid from anisic acid, vanillic acid from veratric acid and syringic acid from trimethoxybenzoic acid. These 4-hydroxybenzoic acids are normally decarboxylated to a high extent after being fed to plants. When they are formed in the plants by O-demethylation they can be isolated partly as free acids but mainly as their glycosides and glucose esters. These observations and some other indications give evidence of a possible compartmentalisation of plant cells.
将在对位和间位甲氧基上特异性标记的各种甲氧基苯甲酸（茴香酸、藜芦酸和3,4,5-三甲氧基苯甲酸）以及相应的4-羟基苯甲酸添加到无菌培养的小麦幼苗的营养液中。实验表明，苯甲酸的O-去甲基化对对位甲氧基具有特异性。间位-O-甲基碳原子仅以非常低的程度以二氧化碳形式出现，并且无法分离出由这些基团去甲基化形成的产物。由对位甲氧基的O-去甲基化形成的产物可以鉴定为：来自茴香酸的对羟基苯甲酸、来自藜芦酸的香草酸和来自三甲氧基苯甲酸的丁香酸。这些4-羟基苯甲酸在被喂给植物后通常会大量脱羧。当它们在植物中通过O-去甲基化形成时，它们可以部分以游离酸的形式分离出来，但主要以其糖苷和葡萄糖酯的形式分离出来。这些观察结果和其他一些迹象证明了植物细胞可能存在区室化。
