Pacheco Larissa Ferreira, Banzi Éfani Caroline de Freitas, Rodrigues Eliana, Soares Luís Eduardo Silva, Pascon Fernanda Miori, Correr-Sobrinho Lourenço, Puppin-Rontani Regina Maria
Department of Pediatric Dentistry, Piracicaba School of Dentistry, UNICAMP - University of Campinas, PiracicabaSP, Brazil.
Department of Dental Materials and Operative Dentistry, Vale do Paraíba University; School of Dentistry, Research and Development Institute, IP&D, Laboratory of Biomedical Vibrational Spectroscopy, LEVB. São José dos CamposSP, Brazil.
Braz Dent J. 2013 Nov-Dec;24(6):610-8. doi: 10.1590/0103-6440201302357.
This study evaluated structural and molecular issues of dentin caries-like lesions produced by different artificial models (ACL) compared with natural caries lesions (NCL). One hundred twenty-four sound occlusal dentin blocks and 47 carious blocks were obtained and surface hardness was analyzed (SH1). They were assigned to groups according to ACL: GB: Biological; GC: Chemical; GIS: In situ; GNC: natural caries (control). Blocks from groups 1, 2 and 3 were submitted to caries lesion induction. NCL and ACL blocks were submitted to surface hardness (SH 2), FT-Raman and µEDXRF analysis. All blocks were longitudinally sectioned and one of the halves was submitted to cross-sectional hardness (CSH) and the other to SEM analysis. SH1 and SH2 data were submitted to t test (unpaired and paired, respectively), CSH and SEM data to two-way and one-way ANOVA respectively, and Tukey and t tests, respectively (p<0.05). Data from FT-Raman/µEDXRF were submitted to one-way ANOVA and Dunnett multiple-comparisons test (a=0.05). GB and GNC showed lowest SH2 values that were significantly different from GC and GIS. Regarding CSH, GB and GNC showed no significant difference between them. SEM showed similar caries lesion depth for GB and GNC, being significantly higher than for GC and GIS. µEDXRF showed similar values of calcium and phosphate for GB and GNC; GNC values were significantly different from GIS. No significant difference was found among the groups concerning phosphate, carbonate and CH bonds values. For collagen type I, GC values were significantly different compared to other groups. It may be concluded that caries-like lesions produced by GB were the closest model to NCL.
本研究评估了不同人工模型（ACL）产生的牙本质龋样病变与自然龋损（NCL）相比的结构和分子问题。获取了124个完好的咬合面牙本质块和47个龋损块，并分析了表面硬度（SH1）。根据人工模型将它们分为不同组：GB组：生物学模型；GC组：化学模型；GIS组：原位模型；GNC组：自然龋损（对照组）。第1、2和3组的块体进行龋损诱导。对NCL和ACL块体进行表面硬度（SH2）、傅里叶变换拉曼光谱（FT-Raman）和微能量色散X射线荧光光谱（µEDXRF）分析。所有块体均纵向切片，其中一半进行横断面硬度（CSH）分析，另一半进行扫描电子显微镜（SEM）分析。SH1和SH2数据分别进行t检验（分别为非配对和配对），CSH和SEM数据分别进行双向和单向方差分析，以及Tukey检验和t检验（p<0.05）。FT-Raman/µEDXRF数据进行单向方差分析和Dunnett多重比较检验（α=0.05）。GB组和GNC组的SH2值最低，与GC组和GIS组有显著差异。关于CSH，GB组和GNC组之间无显著差异。SEM显示GB组和GNC组的龋损深度相似，显著高于GC组和GIS组。µEDXRF显示GB组和GNC组的钙和磷值相似；GNC组的值与GIS组有显著差异。各组之间在磷酸盐、碳酸盐和CH键值方面未发现显著差异。对于I型胶原蛋白，GC组的值与其他组有显著差异。可以得出结论，GB组产生的龋样病变是最接近NCL的模型。
