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甘蔗糖蜜作为枯草芽孢杆菌生物生产色氨酸的前体来源。

Cane molasses as a source of precursors in the bioproduction of tryptophan by Bacillus subtilis.

作者信息

Shasaltaneh Marzieh Dehghan, Moosavi-Nejad Zahra, Gharavi Sara, Fooladi Jamshid

机构信息

Department of Biology, Faculty of Basic Sciences, Alzahra University, Tehran, Iran.

出版信息

Iran J Microbiol. 2013 Sep;5(3):285-92.

PMID:24475338
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3895569/
Abstract

BACKGROUND AND OBJECTIVES

The essential amino acid L-tryptophan can be produced by a condensation reaction between indole and L-serine, catalyzed by B. subtilis with tryptophan synthase activity. Application of the tryptophan is widespread in the biotechnology domain and is sometimes added to feed products as a food fortifier.

MATERIALS AND METHODS

The optimum concentration of the Iranian cane molasses was determined by measuring the amount of biomass after growth in 1 to 30 g/mL of molasses. The maximum amount of biomass was obtained in 10 g/mL molasses. Chromatographic methods, TLC and HPLC, were used to assay the amount of tryptophan produced in the presence of precursors of tryptophan production (indole and serine) and/or molasses.

RESULTS

Our results indicate the importance of the Iranian cane molasses not only as carbon source, but also as a source of precursors for tryptophan production.

CONCLUSION

This report evaluates the potential of cane molasses as an economical source for tryptophan production by B. subtilis, hence eliminating the requirement for additional serine and indole as precursors.

摘要

背景与目的

必需氨基酸L-色氨酸可由吲哚与L-丝氨酸之间的缩合反应产生，该反应由具有色氨酸合酶活性的枯草芽孢杆菌催化。色氨酸在生物技术领域应用广泛，有时作为食品强化剂添加到饲料产品中。

材料与方法

通过测量在1至30 g/mL糖蜜中生长后的生物量来确定伊朗甘蔗糖蜜的最佳浓度。在10 g/mL糖蜜中获得了最大生物量。采用色谱方法，即薄层色谱法（TLC）和高效液相色谱法（HPLC），来测定在色氨酸生产前体（吲哚和丝氨酸）和/或糖蜜存在的情况下产生的色氨酸量。

结果

我们的结果表明，伊朗甘蔗糖蜜不仅作为碳源很重要，而且作为色氨酸生产前体的来源也很重要。

结论

本报告评估了甘蔗糖蜜作为枯草芽孢杆菌生产色氨酸的经济来源的潜力，从而无需额外添加丝氨酸和吲哚作为前体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b004/3895569/4bc65839d99e/IJM-5-285-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b004/3895569/adc850f0abb7/IJM-5-285-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b004/3895569/3225006ff26b/IJM-5-285-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b004/3895569/21d24ea4d8fe/IJM-5-285-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b004/3895569/966e8913ae91/IJM-5-285-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b004/3895569/0183cdc37016/IJM-5-285-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b004/3895569/0f0724160855/IJM-5-285-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b004/3895569/4bc65839d99e/IJM-5-285-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b004/3895569/adc850f0abb7/IJM-5-285-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b004/3895569/3d6dd47529b6/IJM-5-285-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b004/3895569/3225006ff26b/IJM-5-285-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b004/3895569/21d24ea4d8fe/IJM-5-285-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b004/3895569/966e8913ae91/IJM-5-285-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b004/3895569/0183cdc37016/IJM-5-285-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b004/3895569/0f0724160855/IJM-5-285-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b004/3895569/4bc65839d99e/IJM-5-285-g008.jpg

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