Happ E, Scalfi-Happ C, Clore G M, Gronenborn A M
Max-Planck-Institut für Biochemie, Martinsried, FRG.
Nucleic Acids Symp Ser. 1987(18):265-8.
The chemical synthesis of a short RNA duplex, to be used for 500 MHz 1H-NMR experiments, is presented. The target oligonucleotide was constructed in a blockwise fashion according to the benzotriazolyl phosphotriester approach in solution. The CPTr group, 4,4',4"-tris(4,5-dichlorophthalimido)trityl, was used for temporary protection of the 5'-OH; this blocking group was removed by buffered hydrazine without harming any other protecting group.
本文介绍了一种用于500兆赫1H - NMR实验的短RNA双链体的化学合成方法。目标寡核苷酸是根据溶液中的苯并三唑基磷酸三酯法以逐步构建的方式进行合成的。使用CPTr基团，即4,4',4"-三(4,5-二氯邻苯二甲酰亚胺)三苯甲基，对5'-OH进行临时保护；该保护基团可通过缓冲肼去除，且不会损害任何其他保护基团。
