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1
Synthesis of short RNA fragments by the benzotriazolyl phosphotriester approach.通过苯并三唑基磷酸三酯法合成短RNA片段。
Nucleic Acids Res. 1983 Dec 10;11(23):8389-405. doi: 10.1093/nar/11.23.8389.
2
Ribonucleoside and ribonucleotide derivatives in polynucleotide synthesis.多核苷酸合成中的核糖核苷和核糖核苷酸衍生物。
Nucleic Acids Symp Ser. 1980(7):99-102.
3
Further studies on oligoribonucleotide synthesis.寡核糖核苷酸合成的进一步研究。
Nucleic Acids Symp Ser. 1980(7):115-27.
4
Solid-phase synthesis of oligoribonucleotides.寡核糖核苷酸的固相合成
Nucleic Acids Symp Ser. 1985(16):173-6.
5
Synthesis of RNA oligomer using 9-fluorenylmethoxycarbonyl (Fmoc) group for 5'-hydroxyl protection.
Nucleic Acids Symp Ser. 1988(19):13-6.
6
Chemical synthesis of r(GCAUGC) for high-resolution 1H-NMR studies.用于高分辨率氢核磁共振研究的r(GCAUGC)的化学合成。
Nucleic Acids Symp Ser. 1987(18):265-8.
7
Use of a 1-hydroxybenzotriazole activated phosphorylating reagent towards the synthesis of short RNA fragments in solution.使用1-羟基苯并三唑活化的磷酸化试剂在溶液中合成短RNA片段。
Nucleic Acids Res. 1986 Jul 25;14(14):5885-900. doi: 10.1093/nar/14.14.5885.
8
Synthesis of oligonucleotides with sequences identical with or analogous to the 3'-end of 16S ribosomal RNA of Escherichia coli: preparation of m-6-2-A-C-C-U-C-C and A-C-C-U-C-m-4-2C via phosphotriester intermediates.合成与大肠杆菌16S核糖体RNA 3'-末端序列相同或相似的寡核苷酸：通过磷酸三酯中间体制备m-6-2-A-C-C-U-C-C和A-C-C-U-C-m-4-2C
Nucleic Acids Res. 1977 Mar;4(3):747-59. doi: 10.1093/nar/4.3.747.
9
Protected deoxyribonucleoside-3' aryl phosphodiesters as key intermediates in polynucleotide synthesis. Construction of an icosanucleotide analogous to the sequence at the ends of Rous sarcoma virus 35S RNA.作为多核苷酸合成关键中间体的受保护脱氧核糖核苷-3'-芳基磷酸二酯。构建与劳氏肉瘤病毒35S RNA末端序列类似的二十核苷酸。
Nucleic Acids Res. 1979 Apr;6(4):1557-70. doi: 10.1093/nar/6.4.1557.
10
Nucleoside 3'-phosphotriesters as key intermediates for the oligoribonucleotide synthesis. IV. New method for removal of 2,2,2-trichloroethyl group and 31P NMR as a new tool for analysis of deblocking of internucleotide phosphate protecting groups.核苷3'-磷酸三酯作为寡核糖核苷酸合成的关键中间体。IV. 去除2,2,2-三氯乙基基团的新方法以及31P NMR作为分析核苷酸间磷酸保护基团脱保护的新工具。
Nucleic Acids Res. 1977 Jul;4(7):2321-9. doi: 10.1093/nar/4.7.2321.

引用本文的文献

1
Preparation of oligonucleotides corresponding to the acceptor stem of yeast tRNAPhe and their interaction with yeast ATP(CTP):tRNA nucleotidyltransferase.对应于酵母苯丙氨酸转运核糖核酸受体茎的寡核苷酸的制备及其与酵母ATP(CTP):tRNA核苷酸转移酶的相互作用
Nucleic Acids Res. 1984 Sep 11;12(17):6909-22. doi: 10.1093/nar/12.17.6909.
2
Polymer supported DNA synthesis using hydroxybenzotriazole activated phosphotriester intermediates.使用羟基苯并三唑活化的磷酸三酯中间体进行聚合物支持的DNA合成。
Nucleic Acids Res. 1984 Nov 26;12(22):8639-51. doi: 10.1093/nar/12.22.8639.
3
Synthesis of nucleic acid methylphosphonates via the 1-hydroxybenzotriazole phosphotriester approach.通过1-羟基苯并三唑磷酸三酯法合成核酸甲基膦酸酯。
Nucleic Acids Res. 1986 Mar 11;14(5):2171-85. doi: 10.1093/nar/14.5.2171.
4
Synthesis of cyclic oligonucleotides by a modified phosphotriester approach.通过改良的磷酸三酯法合成环状寡核苷酸。
Nucleic Acids Res. 1988 May 25;16(10):4607-20. doi: 10.1093/nar/16.10.4607.
5
Preparation of covalently linked DNA-RNA hybrids and arabinocytidine containing DNA fragments.共价连接的DNA-RNA杂交体及含阿糖胞苷的DNA片段的制备。
Nucleic Acids Res. 1988 Apr 11;16(7):2987-3003. doi: 10.1093/nar/16.7.2987.
6
Use of a 1-hydroxybenzotriazole activated phosphorylating reagent towards the synthesis of short RNA fragments in solution.使用1-羟基苯并三唑活化的磷酸化试剂在溶液中合成短RNA片段。
Nucleic Acids Res. 1986 Jul 25;14(14):5885-900. doi: 10.1093/nar/14.14.5885.

本文引用的文献

1
Further studies on oligoribonucleotide synthesis.寡核糖核苷酸合成的进一步研究。
Nucleic Acids Symp Ser. 1980(7):115-27.
2
The use of high-performance liquid chromatography in the isolation and analysis of oligoribonucleotides synthesized by the T4 RNA ligase reaction.高效液相色谱法在通过T4 RNA连接酶反应合成的寡核糖核苷酸的分离与分析中的应用。
Anal Biochem. 1982 Jul 15;124(1):37-44. doi: 10.1016/0003-2697(82)90216-0.
3
A study of the efficiency and the problem of sulfonation of several condensing reagents and their mechanisms for the chemical synthesis of deoxyoligoribonucleotides.几种缩合试剂磺化用于化学合成脱氧寡核糖核苷酸的效率、问题及其机制的研究
Nucleic Acids Res. 1980 Nov 25;8(22):5445-59. doi: 10.1093/nar/8.22.5445.
4
The case of sulfonation in the chemical synthesis of oligonucleotides.寡核苷酸化学合成中的磺化反应实例。
Nucleic Acids Res. 1980 May 24;8(10):2301-5. doi: 10.1093/nar/8.10.2301.
5
Conformational analysis of the single-stranded ribonucleic acid A-A-C-C. A one-dimensional and two-dimensional proton NMR study at 500 MHz.单链核糖核酸A - A - C - C的构象分析。500兆赫下的一维和二维质子核磁共振研究。
Eur J Biochem. 1983 Apr 5;131(3):571-9. doi: 10.1111/j.1432-1033.1983.tb07301.x.
6
High-pressure liquid chromatography in polynucleotide synthesis.多核苷酸合成中的高压液相色谱法。
Biochemistry. 1978 Apr 4;17(7):1257-67. doi: 10.1021/bi00600a020.
7
Synthesis of oligonucleotides with sequences identical with or analogous to the 3'-end of 16S ribosomal RNA of Escherichia coli: preparation of A-C-C-U-C-C via the modified phosphotriester method.合成与大肠杆菌16S核糖体RNA 3'-末端序列相同或相似的寡核苷酸：通过改良的磷酸三酯法制备A-C-C-U-C-C。
Nucleic Acids Res. 1977 Apr;4(4):1047-63. doi: 10.1093/nar/4.4.1047.

通过苯并三唑基磷酸三酯法合成短RNA片段。

Synthesis of short RNA fragments by the benzotriazolyl phosphotriester approach.

作者信息

Wreesmann C T, Fidder A, van der Marel G A, van Boom J H

出版信息

Nucleic Acids Res. 1983 Dec 10;11(23):8389-405. doi: 10.1093/nar/11.23.8389.

DOI:10.1093/nar/11.23.8389
PMID:6200828
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC326589/
Abstract

Analytical and experimental evidence will be presented to show that addition of a slight excess of 2-chlorophenyl-0,0-bis[1-benzotriazolyl]phosphate to a properly 2',5'-protected ribonucleoside gives an intermediate 3'-phosphotriester, which, after addition of a 2'-protected ribonucleoside, affords a dimer containing solely a 3'-5'-internucleotide linkage. The above phosphorylation procedure has been used in the synthesis of the pentamer UpApCpGpC.

摘要

将给出分析和实验证据，以表明向适当的2'，5'-保护的核糖核苷中加入略微过量的2-氯苯基-0,0-双[1-苯并三唑基]磷酸酯会生成中间体3'-磷酸三酯，在加入2'-保护的核糖核苷后，得到仅含有3'-5'-核苷酸间连接的二聚体。上述磷酸化方法已用于五聚体UpApCpGpC的合成。