Fukuda T, Hamana T, Marumoto R
Biotechnology Laboratories, Central Research Division, Takeda Chemical Industries Ltd, Osaka, Japan.
Nucleic Acids Symp Ser. 1988(19):13-6.
An approach using a new combination of protecting groups in RNA oligomer synthesis is proposed, in which 5'-hydroxyl group of ribose moiety is temporarily protected with the alkaline labile 9-fluorenylmethoxycarbonyl (Fmoc) group and the 2'-hydroxyl group is protected with the acid labile 1-ethoxyethyl (EE) group. The adoption of this method presented great selectivity in removing the 5'-hydroxyl protecting group and facilitated the RNA oligomer synthesis. A RNA pentamer was synthesized by the phosphotriester method in solution.
