Bourel D, Fauchet R, Dejour G, Bouhallier O, Merdrignac G, Chales G, Genetet B
Centre Régional de Transfusion Sanguine, Rennes, France.
Tissue Antigens. 1987 Sep;30(3):97-103. doi: 10.1111/j.1399-0039.1987.tb01604.x.
An anti-HLA-B27 monoclonal antibody produced by the hybridoma technique is described. This BD.7 reagent is a cytotoxic IgM antibody. Its reactivity was studied by lymphocytotoxicity tests, indirect immunofluorescence tests and biochemical analysis against an extensive panel of peripheral blood mononuclear cells. All HLA-B27 positive samples, either from normal subjects or from patients with Ankylosing Spondylitis, were recognized by this reagent. Moreover, a cross-reaction was observed with HLA-B13 cells, and a new unexpected reaction with all HLA-B37 cell suspensions. The interest of such a reagent is discussed.
