Hansen T, Bratlie A, Hannestad K
Institute of Medical Biology, University of Tromsø School of Medicine, Norway.
Tissue Antigens. 1988 Nov;32(5):267-77. doi: 10.1111/j.1399-0039.1988.tb01666.x.
PBMC were isolated from a multiparous woman with HLA-B27 specific Abs in her serum. The HLA type of the donor was A2,9:B7. The PBMC were EBV transformed, and four cell lines making cytotoxic Abs to HLA-B27+ cells prepared. Hybridomas were constructed by fusing the EBV lines with the human fusion partner KR4. All four mAbs were of IgM isotype. One mAb (TrBH12) reacted specifically with B27+, B37+ and Bw47+ lymphoblastoid cell lines and with all B27+ PBMC except for a rare variant so far found only in one Norwegian family. Another mAb (Tr3B6) was cytotoxic for all B27+ cells tested, including the TrBH12- variant; in addition, it showed weaker cross-reactions to Bw42, B49 and a cell line with the probable phenotype B7,38. Supernatant from the Tr3B6 hybridoma was tested in lymphocytotoxicity against a panel of 658 individuals, 141 of whom were B27+. With this panel, Tr3B6 showed perfect correlation with HLA-B27. The two last mAbs (TrCG10 and TrBF1) reacted with all B27+ cells tested, but in addition showed quite extensive cross-reactions.
从一名血清中含有HLA - B27特异性抗体的经产妇中分离出外周血单个核细胞（PBMC）。供体的HLA类型为A2,9:B7。PBMC经EB病毒转化，制备了四种对HLA - B27 +细胞产生细胞毒性抗体的细胞系。通过将EB病毒系与人融合伙伴KR4融合构建杂交瘤。所有四种单克隆抗体均为IgM同种型。一种单克隆抗体（TrBH12）与B27 +、B37 +和Bw47 +淋巴母细胞系以及除了迄今为止仅在一个挪威家族中发现的罕见变体之外的所有B27 + PBMC特异性反应。另一种单克隆抗体（Tr3B6）对所有测试的B27 +细胞具有细胞毒性，包括TrBH12变体；此外，它对Bw42、B49和一个可能具有B7,38表型的细胞系表现出较弱的交叉反应。用Tr3B6杂交瘤的上清液对一组658名个体进行淋巴细胞毒性测试，其中141名是B27 +。对于该组，Tr3B6与HLA - B27显示出完美的相关性。最后两种单克隆抗体（TrCG10和TrBF1）与所有测试的B27 +细胞反应，但此外还表现出相当广泛的交叉反应。
