Weikel W, Wagner R, Moll R
Institute of Pathology, University of Mainz, Federal Republic of Germany.
Virchows Arch B Cell Pathol Incl Mol Pathol. 1987;54(2):98-110. doi: 10.1007/BF02899201.
We have analyzed the expression of cytokeratin polypeptides in subcolumnar reserve cells of the human uterine endocervical mucosa and the other epithelial cells using immunoperoxidase and immunofluorescence microscopy as well as by applying two-dimensional gel electrophoresis to microdissected cytoskeletal preparations. Endocervical columnar cells were uniformly positive for antibodies directed against the simple epithelium-type cytokeratins nos. 7, 8, 18, and 19, while a variable proportion of these cells was stained by an antibody against cytokeratin no. 4. Reserve cells were not only positive for cytokeratins nos. 8 (weakly and variably) and 19 but were also decorated by antibody KA 1, which reacts with cytokeratins present in stratified squamous epithelia. This last antibody selectively decorated reserve cells even when they were flat and inconspicuous. Antibody KA 1 uniformly stained the ectocervical squamous epithelium, the basal cells of which were also decorated by antibodies directed against cytokeratins nos. 8 (weakly and variably) and 19. Ectocervical suprabasal cells were positive, to a variable extent, for antibodies against cytokeratins nos. 4, 10/11, and 13. Gel electrophoresis revealed the presence of squamous-type cytokeratins nos. 5 and 17 in reserve cell-rich, but not in reserve cell-free, endocervical mucosa. We also analyzed the distribution pattern of these cells, as revealed by antibody KA 1, in the endocervical mucosa of 26 uteri. In all the specimens examined reserve cells were present, but their numbers exhibited considerable variation. In some cases these cells were confined to small islets localized deep within the cervical canal and lacked any continuity with the squamous epithelium. The expression of cytokeratins nos. 5 and 17 in reserve cells indicates that these cells have undergone a low level of squamous differentiation. The additional expression of cytokeratins nos. 8 and 19 in these cells points to a relationship with simple epithelial cells. The present data would seem to favor the view that reserve cells originate in situ from the columnar epithelium; however, this would imply an acquisition of new differentiation properties.
我们运用免疫过氧化物酶和免疫荧光显微镜技术，以及对显微切割的细胞骨架制剂进行二维凝胶电泳，分析了人子宫颈内膜黏膜柱状下储备细胞及其他上皮细胞中细胞角蛋白多肽的表达情况。子宫颈柱状细胞对针对简单上皮型细胞角蛋白7、8、18和19的抗体呈均匀阳性反应，而这些细胞中有可变比例被抗细胞角蛋白4的抗体染色。储备细胞不仅对细胞角蛋白8（弱阳性且变化不定）和19呈阳性反应，还被与分层鳞状上皮中存在的细胞角蛋白发生反应的抗体KA 1标记。即使储备细胞扁平且不显眼，最后一种抗体也能选择性地标记它们。抗体KA 1均匀地染色宫颈外鳞状上皮，其基底细胞也被针对细胞角蛋白8（弱阳性且变化不定）和19的抗体标记。宫颈外基底上层细胞对针对细胞角蛋白4、10/11和13的抗体呈不同程度的阳性反应。凝胶电泳显示，富含储备细胞的子宫颈内膜黏膜中存在鳞状细胞型细胞角蛋白5和17，而无储备细胞的黏膜中则不存在。我们还分析了抗体KA 1显示的这些细胞在26个子宫的子宫颈内膜黏膜中的分布模式。在所有检查的标本中都存在储备细胞，但它们的数量有很大差异。在某些情况下，这些细胞局限于宫颈管深处的小胰岛，与鳞状上皮没有任何连续性。储备细胞中细胞角蛋白5和17的表达表明这些细胞已经经历了低水平的鳞状分化。这些细胞中细胞角蛋白8和19的额外表达表明它们与简单上皮细胞有关。目前的数据似乎支持储备细胞原位起源于柱状上皮的观点；然而，这意味着获得了新的分化特性。
