Nagle R B, Moll R, Weidauer H, Nemetschek H, Franke W W
Differentiation. 1985;30(2):130-40. doi: 10.1111/j.1432-0436.1985.tb00524.x.
The distribution and type of cytokeratins present in the normal human epithelia of the nasopharynx, oropharynx, tongue, palatine tonsil, epiglottis, vocal cord, and laryngeal ventricle were studied using immunohistochemical techniques and by gel electrophoresis of cytoskeletal proteins microdissected from frozen tissues. Noncornifying stratified epithelia covering the oropharynx, tongue, surface of the palatine tonsil, pharyngeal surface of the epiglottis, and vocal cord were all found to contain cytokeratins nos. 4, 5, 6, 13, 14, and 15, together with minor amounts of cytokeratin no. 19, i.e., a pattern similar to that previously reported for esophageal epithelium. The immunohistochemical reaction with KA4, an antibody specific for cytokeratins nos. 14, 15, 16, and 19, revealed reactivity confined to the basal epithelial cells of the tongue, oropharynx, pharyngeal epiglottis, and two out of five samples of vocal cords. This same antibody reacted with the entire thickness of three out of the five true vocal cords which were shown by gel electrophoresis to also contain cytokeratins nos. 16 and 17. Gel electrophoresis revealed that the pseudostratified columnar epithelium covering the laryngeal ventricle was more complex, in that it contained cytokeratins nos. 5, 13, 14, 15, and 17, which are typical of stratified epithelia, as well as cytokeratins nos. 7, 8, 18, and 19, which are characteristic of simple epithelia. This pattern is similar to that found in bronchial epithelium. The laryngeal surface of the epiglottis exhibited cytokeratins nos. 4, 5, 7, 8, 13, 14, 15, 17, 18, and 19, i.e., a pattern combining features of both esophageal- and bronchial-type epithelia. The reaction of these epithelia containing columnar cells with antibody RGE-53, which is specific for cytokeratin no. 18, revealed a staining reaction confined to the superficial columnar cells, whereas KA1 stained only the basal cells of these epithelia. The results of our study make it possible to distinguish two types of noncornifying stratified squamous epithelium, namely the 'esophageal type' which covers the tongue, oropharynx, and pharyngeal surface of the epiglottis, and another type which overlies the vocal cords and the transitional zone between the pharyngeal and laryngeal surfaces of the epiglottis. Furthermore, there appear to be variants of pseudostratified columnar epithelium, i.e., the usual bronchial type lining the laryngeal ventricle, and a type with a thicker subcolumnar cell compartment that is found on the laryngeal surface of the epiglottis. The patterns of expression of cytokeratins in the respiratory tract are compared with those of other epithelia.
采用免疫组织化学技术以及对从冷冻组织中显微切割的细胞骨架蛋白进行凝胶电泳的方法，研究了细胞角蛋白在正常人体鼻咽、口咽、舌、腭扁桃体、会厌、声带及喉室上皮中的分布和类型。发现覆盖口咽、舌、腭扁桃体表面、会厌咽面及声带的非角化复层上皮均含有细胞角蛋白4、5、6、13、14和15，以及少量的细胞角蛋白19，即与先前报道的食管上皮相似的模式。用KA4（一种对细胞角蛋白14、15、16和19特异的抗体）进行免疫组织化学反应，结果显示反应局限于舌、口咽、会厌咽面及五分之二的声带样本的基底上皮细胞。同一抗体与五分之三的真声带全层发生反应，凝胶电泳显示这些真声带也含有细胞角蛋白16和17。凝胶电泳显示覆盖喉室的假复层柱状上皮更为复杂，因为它含有复层上皮典型的细胞角蛋白5、13、14、15和17，以及单层上皮特征性的细胞角蛋白7、8、18和19。这种模式与支气管上皮中发现的模式相似。会厌喉面表现出细胞角蛋白4、5、7、8、13、14、15、17、18和19，即一种兼具食管型和支气管型上皮特征的模式。这些含有柱状细胞的上皮与对细胞角蛋白18特异的抗体RGE - 53反应，显示染色反应局限于表层柱状细胞，而KA1仅对这些上皮的基底细胞染色。我们的研究结果使得区分两种非角化复层鳞状上皮成为可能，即覆盖舌、口咽和会厌咽面的“食管型”，以及覆盖声带与会厌咽面和喉面之间过渡区的另一种类型。此外，似乎存在假复层柱状上皮的变体，即覆盖喉室的常见支气管型，以及在会厌喉面发现的具有较厚亚柱状细胞区的类型。将呼吸道中细胞角蛋白的表达模式与其他上皮的模式进行了比较。
