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[用光学显微镜进行幽门弯曲菌组织学显示的变体]

[Variants for the histological demonstration of pyloric Campylobacter with optical microscopy].

作者信息

Zárate J O, Santos Lucero R, Padorno L O, Espiniella F, Apud A

机构信息

H.A.C. Hospital Aeronáutico Central Servicios de Patología y Gastroenterología, Buenos Aires, República Argentina.

出版信息

Acta Gastroenterol Latinoam. 1987;16(4):233-40.

PMID:2447734
Abstract

It is analyzed the use of different staining and observation techniques of Pyloric Campylobacter in gastric biopsies and brushings. Toe the ones already conventional of GRAM and W-S, the following are added: W-S + A.B.; P.A.S. + W-S; W-S; W-S + H-E; PAPANICOLAOU, MASSON'S TRICHROMIC, GRAM in tissues and complements of observation with polarization phase contrast and fluorescence. The advantages and applications connected with the bacterium study (presence, morphology, identification, quantity, location) and alterations of the antral mucosa correlated to it (cellular lesions, mucino-genesis alterations, inflammatory component) of each of them are defined.

摘要

分析了幽门弯曲菌在胃活检组织和刷取物中的不同染色及观察技术的应用。除了已有的常规革兰氏染色和W-S染色外,还增加了以下方法:W-S+抗生物素蛋白-生物素;过碘酸希夫氏染色(P.A.S.)+W-S;W-S;W-S+苏木精-伊红染色(H-E);巴氏染色、马森三色染色、组织中的革兰氏染色以及用偏振相差显微镜和荧光显微镜进行补充观察。明确了每种方法在细菌研究(存在情况、形态、鉴定、数量、位置)以及与之相关的胃窦黏膜改变(细胞病变、黏液生成改变、炎症成分)方面的优势和应用。

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