Department of Histology and Embryology, Jining Medical University, Shandong Province, People's Republic of China.
Eur Arch Otorhinolaryngol. 2014 Jun;271(6):1653-9. doi: 10.1007/s00405-014-2903-z. Epub 2014 Jan 30.
Luteolin, a naturally occurring flavonoid, possesses anti-cancer activities against several human cancers, but the exact molecular and biochemical mechanisms of above findings are not very clear, and its activity against head and neck squamous cell carcinoma (HNSCC) is seldom mentioned. In this study, we investigated luteolin against human laryngeal squamous cell line Hep-2 cells, using MTT assay, flow cytometry, Western blot and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. Luteolin inhibited Hep-2 cells proliferation at the inhibitive concentrations of 50% (IC50) near to 50 μM and induced the apoptosis in Hep-2 cells through caspase-3 and caspase-8 activation. Up-regulation of Fas and down-regulation of long form cellular FLICE-like inhibitory protein (c-FLIPL) protein were also involved after luteolin treatment at both protein and mRNA levels. Luteolin could not only inhibit cell proliferation but also induce apoptosis by activating the Fas signaling pathway at the receptor level in laryngeal squamous cell line Hep-2 cells.
木樨草素是一种天然存在的类黄酮，对多种人类癌症具有抗癌活性，但上述发现的确切分子和生化机制尚不清楚，其对头颈鳞状细胞癌（HNSCC）的活性很少被提及。在这项研究中，我们使用 MTT 测定法、流式细胞术、Western blot 和逆转录-聚合酶链反应（RT-PCR）分析研究了木樨草素对人喉鳞状细胞系 Hep-2 细胞的作用。木樨草素在抑制浓度为 50%（IC50）接近 50 μM 时抑制 Hep-2 细胞的增殖，并通过 caspase-3 和 caspase-8 的激活诱导 Hep-2 细胞凋亡。木樨草素处理后，Fas 的上调和长形式细胞 FLICE 样抑制蛋白（c-FLIPL）蛋白的下调也同时发生在蛋白和 mRNA 水平上。木樨草素不仅可以通过在喉鳞状细胞系 Hep-2 细胞的受体水平上激活 Fas 信号通路来抑制细胞增殖，还可以诱导细胞凋亡。
