Ecotoxicology Research Center, Korea Institute of Toxicology, Daejeon, 305-343, Republic of Korea.
Bull Environ Contam Toxicol. 2014 May;92(5):540-5. doi: 10.1007/s00128-014-1213-x. Epub 2014 Jan 30.
In this study, antioxidant responses including lipid peroxidation (LPO), superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST), were evaluated in the liver, gill and muscle tissues of pale chub (Zacco platypus) exposed to copper (Cu) and benzo[a]pyrene (BaP). Cu exposure induced significant antioxidant responses in Z. platypus, particularly in the liver, whereas BaP exposure had a negligible effect. Following Cu exposure, both SOD and CAT activity increased in a concentration-dependent manner, showing significant correlations with malondialdehyde (MDA) levels as a measure of LPO (r = 0.646 and 0.663, respectively). SOD, CAT and GST mRNA levels were also enhanced following Cu exposure, except at 20 μg L(-1), although significant correlations with antioxidant enzyme activities were not found. The results of this study suggest that combined information on SOD and CAT activities together with LPO levels in the liver could be a useful indicator for assessing oxidative stress in freshwater fish.
在这项研究中，评估了抗氧化反应，包括脂质过氧化 (LPO)、超氧化物歧化酶 (SOD)、过氧化氢酶 (CAT) 和谷胱甘肽 S-转移酶 (GST)，在铜 (Cu) 和苯并[a]芘 (BaP) 暴露下的白鲦 (Zacco platypus) 的肝脏、鳃和肌肉组织中。Cu 暴露诱导了 Z. platypus 中显著的抗氧化反应，特别是在肝脏中，而 BaP 暴露则几乎没有影响。Cu 暴露后，SOD 和 CAT 活性均呈浓度依赖性增加，与丙二醛 (MDA) 水平作为 LPO 的衡量指标呈显著相关 (r = 0.646 和 0.663)。Cu 暴露后 SOD、CAT 和 GST mRNA 水平也升高，除了在 20 μg L(-1) 时，尽管与抗氧化酶活性没有显著相关性。本研究结果表明，结合肝脏中 SOD 和 CAT 活性以及 LPO 水平的信息，可能是评估淡水鱼类氧化应激的有用指标。
