Green K, DeBarge L R, Cheeks L, Phillips C I
Department of Ophthalmology, Medical College of Georgia, Augusta.
Acta Ophthalmol (Copenh). 1987 Oct;65(5):538-44. doi: 10.1111/j.1755-3768.1987.tb07037.x.
The centripetal movement of fluorescein and fluorescein-labelled dextrans (4 to 150 kD) from sclera or cut edge of the cornea was determined in isolated rabbit corneas at 4 and 24 h. Corneas were divided into 5.5 mm diameter central core, inner 5.5 to 8 mm donut, 8 to 12 mm peripheral donut and, where applicable, scleral rim. For all molecules greater than sodium fluorescein (376 D) tracer concentrations in the 5.5 mm core and the 5.5 to 8 mm donut were equal. Without sclera rim, the more central portions of the cornea (5.5 mm core and 5.5 to 8 mm donut) had tracer concentrations equal to those of corneas-with-sclera for all tracers greater than 10 kD. The tracer concentrations in the central cornea were the same in the presence or absence of sclera. The data indicate a physiological barrier to the lateral diffusion of molecules greater than 10 kD between the peripheral and more central cornea.
在离体兔角膜中，于4小时和24小时测定了荧光素及荧光素标记的葡聚糖（4至150千道尔顿）从巩膜或角膜切缘的向心运动。角膜被分为直径5.5毫米的中央核心区、内侧5.5至8毫米的环形区、8至12毫米的周边环形区，以及（如适用）巩膜边缘区。对于所有大于荧光素钠（376道尔顿）的分子，5.5毫米核心区和5.5至8毫米环形区中的示踪剂浓度相等。在没有巩膜边缘区的情况下，对于所有大于10千道尔顿的示踪剂，角膜更中央的部分（5.5毫米核心区和5.5至8毫米环形区）的示踪剂浓度与有巩膜的角膜相同。无论有无巩膜，中央角膜中的示踪剂浓度均相同。数据表明，大于10千道尔顿的分子在周边角膜和更中央角膜之间的侧向扩散存在生理屏障。
