Department of Botany, The University, Hull.
Planta. 1972 Sep;107(3):217-26. doi: 10.1007/BF00397937.
Using the aphid stylet technique (14)C ATP was shown to be readily taken up into the sieve elements of willow. At the same time this compound was found to be metabolised during uptake resulting in labelled ADP and AMP appearing in the stylet exudate. Longitudinal movement of labelled ATP was also found to occur.Measurement of the levels of ATP and ADP in stylet exudate showed that both were present in high concentrations. The ratio ATP/ADP varied between 2.0 and 5.3.The effect of certain inhibitors of oxidative phosphorylation (oligomycin and DNP) and glycolysis (fluoride) on the rate of stylet exudation was studied. All three inhibitors caused a cessation of exudation but this did not occur until several hours after inhibitor application. Oligomycin and DNP had no effect on the concentration of ATP in the sap. Fluoride however, appeared in some cases to reduce the ATP concentration to a low level an hour or more before exudation finally stopped.Incorporation of (32)Pi into organic phosphate esters present in stylet exudate was found to occur within 15 minutes of the application of the tracer to a bark strip. Labelling of organic phosphates also took place, at a slower rate, when (32)P inorganic phosphate was incubated with stylet exudate.
采用蚜虫口针刺技术表明，（14）C-ATP 很容易被柳树的筛管吸收。同时，在吸收过程中发现该化合物被代谢，导致标记的 ADP 和 AMP 出现在口针分泌物中。还发现标记的 ATP 会进行纵向移动。测量口针分泌物中的 ATP 和 ADP 水平表明，两者都以高浓度存在。ATP/ADP 的比值在 2.0 到 5.3 之间变化。研究了某些氧化磷酸化抑制剂（寡霉素和 DNP）和糖酵解抑制剂（氟化物）对口针分泌率的影响。这三种抑制剂都导致分泌停止，但直到抑制剂应用数小时后才发生。寡霉素和 DNP 对汁液中的 ATP 浓度没有影响。然而，在口针分泌最终停止前一个多小时，氟化物似乎会将 ATP 浓度降低到一个低水平。在将示踪剂施加到树皮条后 15 分钟内，发现（32）Pi 掺入口针分泌物中存在的有机磷酸酯中。当（32）P 无机磷酸盐与口针分泌物孵育时，也会以较慢的速度标记有机磷酸盐。
