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[软叶丝兰和三蕊柳筛管汁液中ATP及其他有机磷化合物的研究]

[Investigations on ATP and other organic phosphorus compounds in the sieve tube sap of Yucca flaccida and Salix triandra].

作者信息

Kluge M, Becker D, Ziegler H

机构信息

Botanisches Institut der Technischen Hochschule Darmstadt, Darmstadt, Deutschland.

出版信息

Planta. 1970 Mar;91(1):68-79. doi: 10.1007/BF00390166.

Abstract
  1. Like the sieve tube sap of dicotyledons analysed so far, the phloem exudate of Yucca flaccida contains a high level of ATP, i.e. 615 μg/ml on the average. ATP concentration in the phloem exudate does not decrease during extended periods of exudation. 2. The pattern of labelled phosphorylated compounds after feeding of (32)PO 4 (---) is essentially similar in the phloem exudate of Yucca flaccida and in the exudate from the mouth parts of aphids feeding on Salix triandra phloem. 3. The label was incorporated very rapidly into most of the phosphorylated organic compounds present in the exudate of Yucca flaccida. in the first phase after feeding of (32)PO 4 (---) only the label in fructose-1,6-diphosphate and possibly the label in ATP was still increasing in relation to the total radioactivity. This increment was correlated to a loss of radioactivity in the inorganic phosphate fraction. 4. Specific radioactivity of inorganic phosphate and ATP in the sieve tube sap of Yucca flaccida increased continuously throughout the experiments while (32)PO 4 (-) was administered externally. This trend continued for some time after removal of the external source of label.
摘要
  1. 与迄今所分析的双子叶植物筛管汁液一样,软叶丝兰的韧皮部渗出液含有高水平的ATP,即平均为615微克/毫升。在长时间渗出过程中,韧皮部渗出液中的ATP浓度不会降低。2. 用(32)PO₄(---)饲喂后,软叶丝兰韧皮部渗出液和以三蕊柳韧皮部为食的蚜虫口器渗出液中标记的磷酸化化合物模式基本相似。3. 标记物非常迅速地掺入软叶丝兰渗出液中存在的大多数磷酸化有机化合物中。在饲喂(32)PO₄(---)后的第一阶段,相对于总放射性,只有果糖-1,6-二磷酸中的标记物以及可能ATP中的标记物仍在增加。这种增加与无机磷酸盐部分放射性的损失相关。4. 在整个实验过程中,当外部施用(32)PO₄(-)时,软叶丝兰筛管汁液中无机磷酸盐和ATP的比放射性持续增加。在去除外部标记源后,这种趋势持续了一段时间。

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