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两种检测方法用于分子测定布鲁里溃疡病患者临床样本中利福平耐药性的比较。

Comparison of two assays for molecular determination of rifampin resistance in clinical samples from patients with Buruli ulcer disease.

作者信息

Jansson Moritz, Beissner Marcus, Phillips Richard Odame, Badziklou Kossi, Piten Ebekalisai, Maman Issaka, Sarfo Fred Stephen, Huber Kristina Lydia, Rhomberg Agata, Symank Dominik, Wagner Magdalena, Wiedemann Franz, Nitschke Jörg, Banla Kere Abiba, Herbinger Karl-Heinz, Adjei Ohene, Löscher Thomas, Bretzel Gisela

机构信息

Department of Infectious Diseases and Tropical Medicine (DITM), University Hospital, Ludwig-Maximilians-University, Munich, Germany.

出版信息

J Clin Microbiol. 2014 Apr;52(4):1246-9. doi: 10.1128/JCM.03119-13. Epub 2014 Jan 29.

DOI:10.1128/JCM.03119-13
PMID:24478404
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3993512/
Abstract

This study evaluates a novel assay for detecting rifampin resistance in clinical Mycobacterium ulcerans isolates. Although highly susceptible for PCR inhibitors in 50% of the samples tested, the assay was 100% M. ulcerans specific and yielded >98% analyzable sequences with a lower limit of detection of 100 to 200 copies of the target sequence.

摘要

本研究评估了一种用于检测临床溃疡分枝杆菌分离株中利福平耐药性的新检测方法。尽管在50%的测试样本中对PCR抑制剂高度敏感，但该检测方法对溃疡分枝杆菌具有100%的特异性，可产生>98%的可分析序列，检测下限为目标序列的100至200个拷贝。

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本文引用的文献

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Implementation of a national reference laboratory for Buruli ulcer disease in Togo.在多哥实施国家布鲁里溃疡病参考实验室。
PLoS Negl Trop Dis. 2013;7(1):e2011. doi: 10.1371/journal.pntd.0002011. Epub 2013 Jan 24.
2
Detection of viable Mycobacterium ulcerans in clinical samples by a novel combined 16S rRNA reverse transcriptase/IS2404 real-time qPCR assay.通过新型16S rRNA逆转录酶/IS2404实时定量PCR联合检测法检测临床样本中活的溃疡分枝杆菌
PLoS Negl Trop Dis. 2012;6(8):e1756. doi: 10.1371/journal.pntd.0001756. Epub 2012 Aug 28.
3
Spontaneous clearance of a secondary Buruli ulcer lesion emerging ten months after completion of chemotherapy--a case report from Togo.化疗结束十个月后出现的继发性布鲁里溃疡病变自发清除——多哥的一例病例报告
PLoS Negl Trop Dis. 2012;6(7):e1747. doi: 10.1371/journal.pntd.0001747. Epub 2012 Jul 31.
4
Real-time PCR and high-resolution melt analysis for rapid detection of Mycobacterium leprae drug resistance mutations and strain types.实时聚合酶链反应和高分辨率熔解曲线分析快速检测麻风分枝杆菌耐药突变和菌株类型。
J Clin Microbiol. 2012 Mar;50(3):742-53. doi: 10.1128/JCM.05183-11. Epub 2011 Dec 14.
5
Spontaneous clearance of Mycobacterium ulcerans in a case of Buruli ulcer.一例布氏溃疡中溃疡分枝杆菌的自发清除
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6
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7
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8
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