利用环介导等温扩增试验快速灵敏检测溃疡分枝杆菌。
Rapid and sensitive detection of Mycobacterium ulcerans by use of a loop-mediated isothermal amplification test.
机构信息
School of Veterinary Sciences, The University of Queensland, Gatton Campus, Gatton, Queensland, Australia.
出版信息
J Clin Microbiol. 2012 May;50(5):1737-41. doi: 10.1128/JCM.06460-11. Epub 2012 Feb 22.
Abstract
This work reports the design and evaluation of a rapid loop-mediated isothermal amplification test for detecting Mycobacterium ulcerans DNA based on the multicopy insertion sequence IS2404. The test is robust and specific with a detection limit equivalent to 20 copies of the target sequence (0.01 to 0.1 genome). The test has potential for the diagnosis of Buruli ulcer under field conditions.
摘要
本研究报告了一种基于多拷贝插入序列 IS2404 的快速环介导等温扩增检测分枝杆菌溃疡 DNA 的设计和评估。该检测方法具有稳健性和特异性，检测限相当于目标序列的 20 个拷贝（0.01 至 0.1 个基因组）。该检测方法有望在现场条件下用于诊断溃疡分枝杆菌病。
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