Gupta Nidhi, Kandimalla Ramesh, Priyanka Kumari, Singh Gagandip, Gill Kiran Dip, Singh Surjit
Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Nehru Hospital, Chandigarh, 160012 India.
Department of Cardiology, Postgraduate Institute of Medical Education and Research, Nehru Hospital, Chandigarh, 160012 India.
Indian J Clin Biochem. 2014 Jan;29(1):69-73. doi: 10.1007/s12291-013-0300-9. Epub 2013 Feb 1.
Dietary and lifestyle factors have been shown to have a profound effect on paraoxonase-1 (PON1) activity. Cigarette smoke has been shown to inhibit its mass and activity where as resveratrol has been shown to enhance it. We exposed hepatoma derived cell line (HepG2) to resveratrol and nicotine in varying doses and measured PON1 enzymatic activity and PON1 gene expression. In addition, total protein content of HepG2 cells was also measured. Resveratrol in a dose of 15 μmol/l or above significantly increased the PON1 enzyme activity (p > 0.001) where as nicotine in a dose of 1 μmol/l or higher significantly reduced it (p < 0.05). The resveratrol in this dose also enhanced the PON1 gene expression whereas nicotine decreased it as compared to controls. However, the protein conent of cells was not changed suggesting that they were not cytotoxic in the doses used. Till date the antioxidant vitamins have shown disappointing results against LDL oxidation and cardiovascular protection. However, the effect of resveratrol on PON1 gene expression and activity was significant, suggesting increase in PON1 activity and enhanced gene expression may be its alternative mechanism for offering protection against cardiovascular disease and may be an potential pharmacological agent which can be used for this.
饮食和生活方式因素已被证明对对氧磷酶-1（PON1）活性有深远影响。已证明香烟烟雾会抑制其质量和活性，而白藜芦醇则会增强其活性。我们将肝癌衍生细胞系（HepG2）暴露于不同剂量的白藜芦醇和尼古丁中，并测量PON1酶活性和PON1基因表达。此外，还测量了HepG2细胞的总蛋白含量。15μmol/l及以上剂量的白藜芦醇显著增加了PON1酶活性（p>0.001），而1μmol/l及以上剂量的尼古丁则显著降低了该活性（p<0.05）。该剂量的白藜芦醇还增强了PON1基因表达，而尼古丁与对照组相比则降低了该表达。然而，细胞的蛋白质含量没有变化，表明所用剂量对细胞没有细胞毒性。迄今为止，抗氧化维生素在对抗低密度脂蛋白氧化和心血管保护方面的结果令人失望。然而，白藜芦醇对PON1基因表达和活性的影响是显著的，这表明PON1活性的增加和基因表达的增强可能是其预防心血管疾病的另一种机制，并且可能是一种可用于此目的的潜在药物制剂。
