Chen K Y, Cheng S
Department of Chemistry Rutgers, State University of New Jersey, New Brunswick 08903.
Biochem Biophys Res Commun. 1988 Jan 15;150(1):185-91. doi: 10.1016/0006-291x(88)90503-7.
Bacillus alcalophilus, an obligately alkalophilic bacterium that grows at pH 11.0, has an intracellular pH of 9.5 or less. Unlike all other living organisms, polyamines (putrescine, spermidine and spermine) in B. alcalophilus, if present, will be largely unprotonated. HPLC analysis indicated that spermidine is the major polyamine in B. alcalophilus, accounting for more than 90% of total polyamines, and the level of spermidine varies during growth. Ornithine decarboxylase activity was not detectable in B. alcalophilus under all conditions examined. When [3H]arginine was added to the culture medium, the radioactivity can be recovered from polyamine pool; the distribution is 3% for putrescine, 94% for spermidine, and 3% for spermine, suggesting the presence of arginine pathway for polyamine biosynthesis. The polyamine transport system in B. alcalphilus appears to be Na+-dependent and is highly sensitive to the inhibition of gramicidin S and valinomycin.
嗜碱芽孢杆菌是一种专性嗜碱细菌，能在pH 11.0的环境中生长，其细胞内pH值为9.5或更低。与所有其他生物不同，嗜碱芽孢杆菌中的多胺（腐胺、亚精胺和精胺）（如果存在的话）大部分将处于未质子化状态。高效液相色谱分析表明，亚精胺是嗜碱芽孢杆菌中的主要多胺，占总多胺的90%以上，且亚精胺水平在生长过程中会发生变化。在所有检测条件下，均未检测到嗜碱芽孢杆菌中的鸟氨酸脱羧酶活性。当向培养基中添加[3H]精氨酸时，放射性可从多胺池中回收；其分布情况为：腐胺占3%，亚精胺占94%，精胺占3%，这表明存在多胺生物合成的精氨酸途径。嗜碱芽孢杆菌中的多胺转运系统似乎依赖于Na+，并且对短杆菌肽S和缬氨霉素的抑制高度敏感。
