Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, Jülich D-52425, Germany.
Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, Jülich D-52425, Germany.
Curr Opin Biotechnol. 2014 Apr;26:148-54. doi: 10.1016/j.copbio.2014.01.005. Epub 2014 Jan 28.
The engineering of microbial strains for the production of small molecules of biotechnological interest is a time-consuming, laborious and expensive process. This can be mostly attributed to the fact that good producers cannot be readily obtained by high-throughput screening approaches since increased product formation usually does not confer a clear phenotype to producing strain variants. Recently, advances were made in the design and construction of genetically encoded RNA aptamer-based or transcription factor-based biosensors for detecting small molecules at the single-cell level. The first promising examples for the application of these molecular biosensors in combination with fluorescent-activated cell sorting as a high-throughput screening device demonstrated the value and potential of these new tools for microbial strain development.
微生物菌株的工程化生产对于生物技术领域的小分子具有重要意义，但这是一个耗时、费力且昂贵的过程。这主要是因为通过高通量筛选方法很难获得良好的生产者，因为增加产物形成通常不会赋予生产菌株变体明显的表型。最近，在设计和构建基于遗传编码 RNA 适体或转录因子的生物传感器方面取得了进展，这些生物传感器可用于在单细胞水平检测小分子。将这些分子生物传感器与荧光激活细胞分选相结合作为高通量筛选设备的首批有前景的应用实例证明了这些新工具在微生物菌株开发方面的价值和潜力。
