Food Safety Key Laboratory of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, 310035, People's Republic of China.
Parasitol Res. 2014 Mar;113(3):1233-8. doi: 10.1007/s00436-014-3762-7. Epub 2014 Jan 31.
To enhance the anti-coccidial effect of dinitolmide and reduce its residual, the dinitolmide/MMT compounds were synthesized by the method of solution intercalation via dinitolmide intercalated into Na + -montmorillonite (Na + -MMT). The structure of compounds was characterized by X-ray diffraction and Fourier transformed infrared. Its anti-coccidial effect was examined by Eimeria tenella infection experiment. One hundred fifty AA broiler chickens were divided into five groups. Chickens were orally inoculated with 5 × 10(4) E. tenella oocysts after dinitolmide was given. Their curative effects were observed. The results showed that intercalated dinitolmide expanded the basal spacing (d 001) of MMT from 12.6 to 15.2 Å. The IR bands of amide group in dinitolmide/MMT were detected at 1,533 cm(-1) which showed that dinitolmide was successfully intercalated into the interlayer spaces of MMT. The dinitolmide/MMT showed higher anti-coccidian oocyst activity compared with dinitolmide (p < 0.05). The dinitolmide/MMT compound can significantly increase body weight gains and reduce bloody diarrhea, lesion score, and oocyst excretion. The anti-coccidia index of dinitolmide/MMT group (165.21) is much higher than dinitolmide group (88.84). The dinitolmide/MMT hybrid systems can be more effective in control of coccidiosis in comparison to dinitolmide.
为了提高地克珠利的抗球虫效果并降低其残留，采用溶液插层法将地克珠利插入 Na + -蒙脱石（Na + -MMT）中合成了地克珠利/MMT 化合物。采用 X 射线衍射和傅里叶变换红外光谱对化合物的结构进行了表征。通过柔嫩艾美耳球虫感染实验考察了其抗球虫效果。将 150 只 AA 肉鸡分为 5 组，地克珠利给药后经口接种 5×10 4 个柔嫩艾美耳球虫卵囊，观察其疗效。结果表明，插层的地克珠利将 MMT 的基面间距（d 001）从 12.6 扩展到 15.2 Å。地克珠利/MMT 的酰胺基团 IR 带在 1533 cm(-1)处被检测到，表明地克珠利成功地插入了 MMT 的层间空间。与地克珠利相比，地克珠利/MMT 表现出更高的抗球虫卵囊活性（p < 0.05）。地克珠利/MMT 化合物能显著提高体重增加，减少血便、病变评分和卵囊排泄。地克珠利/MMT 组的抗球虫指数（165.21）远高于地克珠利组（88.84）。与地克珠利相比，地克珠利/MMT 混合体系在控制球虫病方面更为有效。
