小鼠骨骼发生组织的全胚胎原位杂交

Whole-mount in situ hybridization on murine skeletogenic tissues.

作者信息

Rutkowsky Timothy, Sharma Deepika, Hilton Matthew J

机构信息

University of Rochester Medical Center, Rochester, NY, USA.

出版信息

Methods Mol Biol. 2014;1130:193-201. doi: 10.1007/978-1-62703-989-5_14.

DOI:10.1007/978-1-62703-989-5_14

PMID:24482174

Abstract

Whole-mount in situ hybridization (WISH) is a highly sensitive method for spatial and temporal visualization of RNA transcripts throughout an entire tissue. This method is an excellent tool for studying both embryonic development and disease. It is based on sequence complementation between a Digoxigenin (DIG)-labeled antisense RNA probe and the sense RNA transcript of interest in the tissue. The labeled probe is then visualized by immunohistochemical methods using an antibody conjugated to alkaline phosphatase that catalyzes a color reaction.

摘要

全胚胎原位杂交（WISH）是一种高度灵敏的方法，用于在整个组织中对RNA转录本进行时空可视化。该方法是研究胚胎发育和疾病的绝佳工具。它基于地高辛配体（DIG）标记的反义RNA探针与组织中感兴趣的有义RNA转录本之间的序列互补。然后，使用与碱性磷酸酶偶联的抗体通过免疫组织化学方法使标记的探针可视化，碱性磷酸酶催化显色反应。