Guloglu M O, Larsen A, Brundin P
Neuronal Survival Unit, Department of Experimental Medical Science, Wallenberg Neuroscience Center, Lund, Sweden.
J Neurosci Res. 2014 May;92(5):564-73. doi: 10.1002/jnr.23355. Epub 2014 Jan 31.
The PA6 stromal cell line comprises a heterogeneous population of cells that can induce both mouse and human embryonic stem cells to differentiate into dopaminergic neurons. This ability of PA6 cells has been termed stromal cell-derived inducing activity (SDIA). The level of SDIA has been found to vary considerably between and within batches of PA6 cells. Not only are the molecular mechanisms that underlie SDIA unknown but also the cell type(s) within the heterogeneous PA6 cultures that underlie SDIA remain poorly defined. In this study, we reveal that adipocytes, which are present within the heterogeneous PA6 cell population, robustly release the factors mediating SDIA. Furthermore, we report that the coculture of human embryonic stem cells with PA6-derived adipocytes reliably induces their differentiation into midbrain dopaminergic neurons.
PA6基质细胞系由一群异质性细胞组成，这些细胞能够诱导小鼠和人类胚胎干细胞分化为多巴胺能神经元。PA6细胞的这种能力被称为基质细胞衍生诱导活性（SDIA）。已发现不同批次以及同一批次内的PA6细胞之间，SDIA水平存在很大差异。不仅SDIA的分子机制尚不清楚，而且异质性PA6培养物中构成SDIA的细胞类型也仍未明确界定。在本研究中，我们发现异质性PA6细胞群体中存在的脂肪细胞能强有力地释放介导SDIA的因子。此外，我们报告称，人类胚胎干细胞与PA6来源的脂肪细胞共培养能可靠地诱导其分化为中脑多巴胺能神经元。
